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Studies on application of primordial germ cells for interspecies chimera and molecular characterization of NANOG in aves
이종간 조류 키메라 생산을 위한 생식세포의 활용 및 NANOG 분석을 통한 생식세포의 특성 규명

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Authors
최희정
Advisor
한재용
Major
농업생명과학대학 농생명공학부
Issue Date
2018-02
Publisher
서울대학교 대학원
Keywords
chickeninterspecies germline chimeraprimordial germ cellsNANOGoviduct
Description
학위논문 (박사)-- 서울대학교 대학원 : 농업생명과학대학 농생명공학부, 2018. 2. 한재용.
Abstract
Avian is one of the excellent models for basic and applied
research such as reproductive system and developmental biology. In
particular, avian primordial germ cells (PGCs) have unique
characteristics and accessibility compared to mammals during early
embryo development. Thus, the production of germline chimera has
been regarded as the most powerful tool via PGCs-mediated
technology. In addition, a detailed understanding of germ cells has
been emphasized for maintaining distinct properties in germ cell
biology. In this regards, we achieved the combination of the unique
migratory route of avian germ cells with an embryo replacement
method could be applied to produce interspecies germline chimeras
and developed potential knowledge for the conservation of wild and
endangered birds through surrogate system. Furthermore, we
investigated the structural characteristics of chicken NANOG
(cNANOG) protein and its biological significance using biochemical
methods and cell assay in PGCs. Also, we revealed the oviduct
specific genes regulated by estrogen for potential therapeutic peptides
or regulation of egg white protein secretion.
Language
English
URI
http://hdl.handle.net/10371/140794
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Theses (Ph.D. / Sc.D._농생명공학부)
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