S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Chemical and Biological Engineering (화학생물공학부) Theses (Master's Degree_화학생물공학부)
Effect of 3D PEG Scaffold Hybridized with MC3T3-E1 Cell-derived Extracellular Matrix on Osteogenesis of Human Mesenchymal Stem Cell
MC3T3-E1 세포주 유래 세포외기질과 융합된 PEG 3차원 지지체의 골분화능
- Nathaniel Hwang
- 공과대학 화학생물공학부
- Issue Date
- 서울대학교 대학원
- Osteogenesis; Mesenchymal stem cell; Extracellular matrix; PEG; Bone regeneration; Tissue engineering; scaffold
- 학위논문 (석사)-- 서울대학교 대학원 : 공과대학 화학생물공학부, 2018. 2. Nathaniel Hwang.
- Existing studies about utilizing extracellular matrix (ECM) has focused on organs obtained from living organisms. But there is limitation in supply of organs has limited supply and the composition and therapeutic efficacy of ECMs has distribution depending on the factors represented as donors health status and age. In contrast, the extracellular matrix obtained from the cell line cultured in the laboratory has the advantage
it could be produced under controlled environment with uniform quality. In this study, we developed an optimized decellularization process to separate the ECM of osteoblast cell line, MC3T3-E1. And we cultured human bone marrow-derived mesenchymal stem cells with the hydrolyzed ECM to evaluate bone-differentiation ability. Osteogenesis of the stem cell was analyzed by ALP staining, alizarin red S staining and real-time PCR. As a result, we confirmed the statistically significant osteoinductivity of the ECM produced by MC3T3-E1 cell line in 2D and 3D condition. Our study suggests that ECM derived from cell lines could be utilized in tissue engineering fields as useful biomaterial.