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Genetic analysis of a Nonpungent EMS mutant in pepper (Capsicum annuum L.)
무신미 EMS 돌연변이체의 유전적 분석

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Authors
이주헌
Advisor
강병철
Major
농업생명과학대학 식물생산과학부
Issue Date
2018-02
Publisher
서울대학교 대학원
Keywords
CapsaicinoidMutMapMutationEMSPungency
Description
학위논문 (석사)-- 서울대학교 대학원 : 농업생명과학대학 식물생산과학부, 2018. 2. 강병철.
Abstract
Capsaicinoid is the alkaloid compounds produced in peppers
(Capsicum spp.). They are responsible for pepper pungency or hotness
and is one of the important traits in breeding programs. Although many
studies have been performed to elucidate its biosynthesis, the
biosynthetic pathway is largely based on studies on the similar pathways
of other plants. To understand the biosynthesis of capsaicinoid, a nonpungent
mutant 221-2-1a, developed from pungent 'Yuwol-cho' were
analyzed. 221-2-1a was found to have no mutation in the coding
sequence of Pun1, but the levels of capsaicinoid in their fruits were
drastically decreased compared to that of Yuwol-cho. To identify the
gene(s) responsible for the non-pungent trait in 221-2-1a. Gene
expressions of 12 genes involved in capsaicinoid biosynthesis were
compared between 221-2-1a and Yuwol-cho together with several
selected cultivars. Seven out of 12 genes (pAMT, BCAT, ACL, KAS, FatA,
PAL, and Pun1) showed a significant decrease in their expression levels
in 221-2-1a compared to pungent cultivars. Furthermore, the inheritance
of pungency was studied in a population derived from a between Yuwolcho
and 221-2-1a. The inheritance study showed that the nonpungency
in 221-2-1a is controlled by two recessive genes. To identify the genes
responsible for non-pungency trait, samples of Yuwol-cho and bulked F3
were sequenced and analyzed by MutMap. A total of 11 SNPs were
identified in the intergenic sequences and the candidates were annotated.
Although candidate genes were not capsaicinoid biosynthesic genes, the
candidate genes are believed to be ideal targets in studies to carryout in
the future.
Language
English
URI
https://hdl.handle.net/10371/141817
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Plant Science (식물생산과학부)Theses (Master's Degree_식물생산과학부)
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