Isolation and Structure Determination of Bioactive Constituents from Poncirus trifoliata Raf.

Abstract

Abstract

Isolation and Structure Determination of Bioactive Constituents from Poncirus trifoliata Raf.

Won-Hee Lee

Natural Products Science Major

College of Pharmacy

Master Course in the Graduate School

Seoul National University

Sortase A (SrtA), a gram-positive transpeptidase, is an important target in reducing bacterial infection. The extract from the fruits of trifoliate orange, Poncirus trifoliata Rafinesque, showed significant Srt A inhibition.

Poncirus trifoliata Raf. is a member of the family Rutaceae and it has been widely used in Korean traditional medicine for the treatment of diverse pathogenic symptoms.

Bioactivity-guided chromatographic separation afforded the isolation of in total twelve compounds, eight coumarins and four flavanone glycosides.

The structures of four novel coumarins were determined by a combination of spectroscopic analyses such as NMR and LC-MS.

The absolute configurations of the new coumarins and known coumarins, previously unassigned, were determined by an application of the 2-methoxy-2-(trifluoromethyl)phenylacetic acid (MTPA) method.

Key words: herbal medicine, Poncirus trifoliata Rafinesque, coumarins, flavanone glycosides, sortase A inhibition

Student number: 2016-29626