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Brefeldin A-sensitive ER-Golgi vesicle trafficking contributes to NLRP3-dependent caspase-1 activation
DC Field | Value | Language |
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dc.contributor.author | Hong, Sujeong | - |
dc.contributor.author | Hwang, Inhwa | - |
dc.contributor.author | Gim, Eunji | - |
dc.contributor.author | Yang, Jungmin | - |
dc.contributor.author | Park, Sangjun | - |
dc.contributor.author | Yoon, Sung-Hyun | - |
dc.contributor.author | Lee, Won-Woo | - |
dc.contributor.author | Yu, Je-Wook | - |
dc.creator | 이원우 | - |
dc.date.accessioned | 2019-04-25T02:08:26Z | - |
dc.date.available | 2020-04-05T02:08:26Z | - |
dc.date.created | 2019-08-29 | - |
dc.date.created | 2019-08-29 | - |
dc.date.issued | 2019-03 | - |
dc.identifier.citation | FASEB Journal, Vol.33 No.3, pp.4547-4558 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://hdl.handle.net/10371/150295 | - |
dc.description.abstract | Endoplasmic reticulum (ER)-Golgi vesicle trafficking plays a pivotal role in the conventional secretory pathway of many cytokines; however, the precise release mechanism of a major inflammasome mediator, IL-1, is not thought to follow the conventional ER-Golgi route and remains elusive. Here, we found that perturbation of ER-Golgi trafficking by brefeldin A (BFA) treatment attenuated nucleotide-binding oligomerization domain-like receptor family, pyrin-domain-containing 3 (NLRP3) inflammasome activation in mouse bone marrow-derived macrophages (BMDMs). BFA treatment inhibited NLRP3-mediated inflammasome assembly and caspase-1 activation but did not block IL-1 secretion from BMDMs following BFA administration after NLRP3 inflammasome activation. Consistently, short-hairpin RNA-dependent knockdown of BFA-inhibited guanine nucleotide-exchange protein 1 (BIG1), a molecular target of BFA and an initiator of Golgi-specific vesicle trafficking, abolished NLRP3-dependent apoptosis-associated speck-like protein containing a caspase-recruitment domain oligomerization and caspase-1 activation in BMDMs. Similarly, knockdown of Golgi-specific BFA-resistance guanine nucleotide exchange factor 1, another target of BFA, clearly attenuated NLRP3-mediated caspase-1 activation in BMDMs. Mechanistically, inhibition of BIG1-mediated vesicle trafficking did not impair NLRP3-activating signal 2-promoted events, such as potassium efflux and mitochondrial rearrangement, but caused significant impairment of signal 1-triggered priming steps, including NF-B-mediated pathways. These data suggest that BFA-targeted vesicle trafficking at the Golgi contributes to activation of the NLRP3 inflammasome signaling.Hong, S., Hwang, I., Gim, E., Yang, J., Park, S., Yoon, S.-H., Lee, W.-W., Yu, J.-W. Brefeldin A-sensitive ER-Golgi vesicle trafficking contributes to NLRP3-dependent caspase-1 activation. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | Federation of American Societies for Experimental Biology | - |
dc.title | Brefeldin A-sensitive ER-Golgi vesicle trafficking contributes to NLRP3-dependent caspase-1 activation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1096/fj.201801585R | - |
dc.citation.journaltitle | FASEB Journal | - |
dc.identifier.wosid | 000459794800115 | - |
dc.identifier.scopusid | 2-s2.0-85067828118 | - |
dc.description.srnd | OAIID:RECH_ACHV_DSTSH_NO:T201826397 | - |
dc.description.srnd | RECH_ACHV_FG:RR00200001 | - |
dc.description.srnd | ADJUST_YN: | - |
dc.description.srnd | EMP_ID:A079475 | - |
dc.description.srnd | CITE_RATE:5.595 | - |
dc.description.srnd | DEPT_NM:의과학과 | - |
dc.description.srnd | EMAIL:wonwoolee@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.citation.endpage | 4558 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 4547 | - |
dc.citation.volume | 33 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Lee, Won-Woo | - |
dc.identifier.srnd | T201826397 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | NLRP3 INFLAMMASOME ACTIVATION | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | INTERLEUKIN-1 | - |
dc.subject.keywordPlus | EXCHANGE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | PYROPTOSOME | - |
dc.subject.keywordPlus | MONOCYTES | - |
dc.subject.keywordAuthor | BFA | - |
dc.subject.keywordAuthor | BIG1 | - |
dc.subject.keywordAuthor | inflammasome | - |
dc.subject.keywordAuthor | IL-1 secretion | - |
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