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Effect of Propionyl-fructooligosaccharides on Fecal Microbiota in ICR Mouse Model : 프로피오닐 프락토올리고당이 ICR 쥐모델의 분변균총에 미치는 영향
DC Field | Value | Language |
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dc.contributor.advisor | 지근억 | - |
dc.contributor.author | 황아경 | - |
dc.date.accessioned | 2019-05-07T04:06:25Z | - |
dc.date.available | 2021-04-13T06:30:20Z | - |
dc.date.issued | 2019-02 | - |
dc.identifier.other | 000000154053 | - |
dc.identifier.uri | https://hdl.handle.net/10371/151323 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 생활과학대학 식품영양학과, 2019. 2. 지근억. | - |
dc.description.abstract | It has been reported that fructooligosaccharides (FOS) has the prebiotic
function and propionic acid is a broadly used food preservative. The aim of the present study was to find the effect of propionyl-fructooligosaccharides P-FOS as a potential prebiotic on fecal microbiota in Institute of Cancer Research (ICR) mouse model. The P-FOS is a novel oligosaccharide product with ester linkage between propionate and FOS. Sixty four 7-week-old specific pathogen free (SPF) grade male ICR mice were administered with different diets into eight groups as follows, ND with normal feed of AIN 93G as control, M with probiotics powder mix in drinking water while maintaining a normal feed, F with 0.5% FOS in normal feed, P with 0.5% P-FOS in normal feed, FM with probiotics powder mix in drinking water and 0.5% FOS in normal feed, PM with probiotics powder mix in drinking water and 0.5% P-FOS in normal feed, FPM with probiotics powder mix in drinking water and normal feed, 0.5% FOS and 0.5% P-FOS in normal feed. The quantitative results of fecal bacteria were analyzed by quantitative real-time PCR (qrt-PCR). The concentration of short chain fatty acid (SCFA) in cecum contents were determined by high performance liquid chromatography. The increases of Bifidobacterium spp. and Lactobacillus spp. in P group with significant difference compared with ND and F group at 5 weeks were shown in the qrt-PCR analysis. Bacteroides spp. was decreased in P group with significant difference compared with F group at 1 and 9 weeks. There was significant increase of Lactobacillus spp. in FP group compared with F and P groups. As for the concentration of SCFA, the greatest increase in the concentration of isobutyrate and total SCFA were shown in PM group. In this study, it was concluded that P-FOS had a better prebiotic effect in improving beneficial bacteria and suppressing some of the opportunistic pathogen bacteria than FOS. In summary, we proved that the P-FOS could be treated as a new functional prebiotic in improving the balance of intestinal microbiota. | - |
dc.description.abstract | P-FOS 는 프락토올리고당(FOS)과 프로피온산의 에스터 결합을
통해 새롭게 합성된 물질로서,선행 연구에서 의해 프락토올리고당의 프리바이오틱스 효과가 입증되었으며, 프로피온산은 식품보존제로 널리 사용되고 있다. 본 연구에서는 새로 합성된 P-FOS 의 섭취가 ICR 마우스의 장내 균총에 미치는 영향을 확인하였다. 본 실험에서 7 주령 수컷 ICR 마우스를 무작위적으로 여덟 군 (n = 8) | - |
dc.description.abstract | 일반식이
섭취군 (ND), 프로바이오틱스 첨가군 (M), 0.5% FOS 첨가군 (F), 0.5% P-FOS 첨가군 (P), 0.5% FOS+0.5% P-FOS 조합군 (FP), 0.5% FOS+프로바이오틱스 조합군 (FM), P-FOS+프로바이오틱스 조합군 (PM), 0.5% FOS+0.5% P-FOS+프로바이오틱스 조합군 (FPM)으로 나누었다. 0, 1, 5, 9 주차에서 분변 샘플을 수집하여 정량 실시간 PCR 을 통해 정량분석 하였다. 또한 9 주차의 맹장내용물을 수집하여 고성능 액체 크로마토그래피를 통해 단쇄지방산 농도를 측정하였다. 실험 결과, FOS 섭취군에 비해 P-FOS 섭취군은 5 주차 에서 Lactobacillus spp., Bifidobacterium spp.는 유의하게 증가하였고, 1, 9주차 에서 장내유해균인 Bacteroides spp. 는 유의하게 감소하였다. FOS+P-FOS 조합된 FP 군은 F 군과 P 군과 비교시, 9 주차 변에서 Lactobacillus spp.가 유의하게 증가하였다. 또한, P-FOS+프로바이오틱스 조합된 PM 군에서 Bifidobacterium spp., Clostridium butyricum 등의 유익균 생육 촉진 및 Bacteroides spp. 등의 유해균 생육 억제 효과가 나타났으며, 다른 군에 비해 PM 군과 FM 군에서 단쇄지방산 이소부티르산 농도가 유의하게 증가하였다. 따라서 새롭게 합성된 P-FOS 는 기존에 상업적으로 사용되는 FOS에 비해 장내 균총의 유익한 변화에 기여하며, 이를 통해 새로운 프리바이오틱스로서의 가능성을 보여주었다. | - |
dc.description.tableofcontents | Contents
Abstract ..................................................................................................... i Table of Contents .................................................................................. iii List of Tables .......................................................................................... v List of Figures ......................................................................................... vi List of Abbreviations ............................................................................ vii 1 Introduction ...................................................................................... 1 2 Materials and Methods .................................................................... 3 2.1 Experimental animals ............................................................... 3 2.2 Preparation of diets ................................................................... 3 2.2.1 Purification of P-FOS ........................................................ 3 2.2.2 Experimental bacteria samples .......................................... 4 2.3 In vivo study design and sample collection .............................. 5 2.4 Microorganisms and culture condition ..................................... 6 2.5 DNA extraction from fecal samples and cultured-bacterial cells ........................................................................................... 7 2.6 Quantitative analysis of fecal microbiota by qrt-PCR .............. 7 2.6.1 Sample preparation for qrt-PCR ........................................ 7 2.6.2 Optimization of qrt-PCR conditions for primers for the quantitative assessment of fecal microbiota .................... 8 2.6.3 Standard curves for qrt-PCR analysis on target bacteria . 10 2.7 Analysis of SCFA concentration ............................................ 11 2.7.1 Cecum specimen preparation .......................................... 11 2.7.2 HPLC sample loading ..................................................... 11 2.8 Statistical analysis .................................................................. 12 3 Results ............................................................................................. 13 3.1 Analysis of P-FOS on TLC .................................................... 13 3.2 Body weight variation ............................................................ 14 3.3 Alteration of fecal microbiota ................................................ 15 3.3.1 Alteration of the number of Lactobacillus spp. ............... 15 3.3.2 Alteration of the number of Bifidobacterium spp. .......... 19 3.3.3 Alteration of the number of Clostridium butyricum ........ 23 3.3.4 Alteration of the number of Bacteroides spp. ................. 27 3.3.5 Alteration of the number of Enterobacter spp. and Escherichia spp. .............................................................. 31 3.4 SCFA concentration in cecum ................................................ 35 4 Discussion ....................................................................................... 37 5 Conclusion ...................................................................................... 40 References .............................................................................................. 41 국문초록........................................................................................... ......45 | - |
dc.language.iso | eng | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject.ddc | 641 | - |
dc.title | Effect of Propionyl-fructooligosaccharides on Fecal Microbiota in ICR Mouse Model | - |
dc.title.alternative | 프로피오닐 프락토올리고당이 ICR 쥐모델의 분변균총에 미치는 영향 | - |
dc.type | Thesis | - |
dc.type | Dissertation | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 생활과학대학 식품영양학과 | - |
dc.date.awarded | 2019-02 | - |
dc.identifier.uci | I804:11032-000000154053 | - |
dc.identifier.holdings | 000000000026▲000000000039▲000000154053▲ | - |
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