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Isolation and characterization of high exopolysaccharide-producing Weissella confusa VP30 from young children’s feces

Cited 6 time in Web of Science Cited 6 time in Scopus
Authors
Jin, Hui; Jeong, Yunju; Yoo, Sang-Ho; Johnston, Tony V; Ku, Seockmo; Ji, Geun Eog
Issue Date
2019-06-13
Publisher
BioMed Central
Citation
Microbial Cell Factories. 18(1):110
Keywords
ExopolysaccharideWeissella confusaDextranLactic acid bacteriaSafetyStructure
Abstract
Background
Lactic acid bacteria (LAB) are known to have a significant ability to colonize the human intestinal tract and adhere to the surface of intestinal epithelial cells. Among the various lactic acid bacteria, exopolysaccharide (EPS) producing strains are known to provide a variety of health benefits for their hosts (e.g. anti-inflammatory, antioxidant, antitumor and stress tolerant effects). Recently, EPSs and EPS-producing lactic acid cultures have gained interest within the food industry and are playing important roles as biothickeners and texturizing agents due to their hydrocolloidal nature. In this study, 156 lactic acid bacterial strains isolated from fecal samples of healthy young children were screened and evaluated for active EPS-production capability.

Results
Among the various human origin lactic acid flora isolated, Weissella confusa VP30 showed the highest EPS productivity and its EPS producing properties were characterized under various cultural conditions in this research. To document the safety of W. confusa VP30, antibiotic resistance, hemolytic, and ammonia production properties were evaluated in addition. No significant negative results were observed. The maximum EPS production by W. confusa VP30 was 59.99 ± 0.91 g/l after 48 h of cultivation in media containing 10% sucrose, far exceeding EPS production by other bacterial strains reported elsewhere. Based on gel permeation chromatography (GPC), the molecular weight of EPS produced by W. confusa VP30 was 3.8 × 106 Da. Structural analysis of the released EPS fraction by 13C and 1H nuclear magnetic resonance (NMR) spectroscopy revealed that W. confusa VP30 can produce dextran with glucose units linked with 96.5% α (1 → 6) glycosidic bonds and 3.5% α (1 → 3) branches.

Conclusion
The high EPS production capability and safety of W. confusa VP30 justify food industry consideration of this cell strain for further evaluation and potential industrial use.
ISSN
1475-2859
Language
English
URI
http://hdl.handle.net/10371/156080
DOI
https://doi.org/10.1186/s12934-019-1158-1
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College of Human Ecology (생활과학대학)Dept. of Food and Nutrition (식품영양학과)Journal Papers (저널논문_식품영양학과)
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