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In silico identification of metabolic engineering strategies for improved lipid production in Yarrowia lipolytica by genome-scale metabolic modeling

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dc.contributor.authorKim, Minsuk-
dc.contributor.authorPark, Beom Gi-
dc.contributor.authorKim, Eun-Jung-
dc.contributor.authorKim, Joonwon-
dc.contributor.authorKim, Byung-Gee-
dc.date.accessioned2019-10-30T00:27:30Z-
dc.date.available2019-10-30T09:38:24Z-
dc.date.issued2019-07-24-
dc.identifier.citationBiotechnology for Biofuels, 12(1):187ko_KR
dc.identifier.issn1754-6834-
dc.identifier.urihttps://hdl.handle.net/10371/162579-
dc.description.abstractBackground
Yarrowia lipolytica, an oleaginous yeast, is a promising platform strain for production of biofuels and oleochemicals as it can accumulate a high level of lipids in response to nitrogen limitation. Accordingly, many metabolic engineering efforts have been made to develop engineered strains of Y. lipolytica with higher lipid yields. Genome-scale model of metabolism (GEM) is a powerful tool for identifying novel genetic designs for metabolic engineering. Several GEMs for Y. lipolytica have recently been developed; however, not many applications of the GEMs have been reported for actual metabolic engineering of Y. lipolytica. The major obstacle impeding the application of Y. lipolytica GEMs is the lack of proper methods for predicting phenotypes of the cells in the nitrogen-limited condition, or more specifically in the stationary phase of a batch culture.

Results
In this study, we showed that environmental version of minimization of metabolic adjustment (eMOMA) can be used for predicting metabolic flux distribution of Y. lipolytica under the nitrogen-limited condition and identifying metabolic engineering strategies to improve lipid production in Y. lipolytica. Several well-characterized overexpression targets, such as diglyceride acyltransferase, acetyl-CoA carboxylase, and stearoyl-CoA desaturase, were successfully rediscovered by our eMOMA-based design method, showing the relevance of prediction results. Interestingly, the eMOMA-based design method also suggested non-intuitive knockout targets, and we experimentally validated the prediction with a mutant lacking YALI0F30745g, one of the predicted targets involved in one-carbon/methionine metabolism. The mutant accumulated 45% more lipids compared to the wild-type.

Conclusion
This study demonstrated that eMOMA is a powerful computational method for understanding and engineering the metabolism of Y. lipolytica and potentially other oleaginous microorganisms.
ko_KR
dc.description.sponsorshipThis research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF2017R1E1A1A01073523) and Industrial Strategic technology development program, 20002734 funded by the Ministry of Trade, Industry & Energy (MI,
Korea)
ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.subjectGenome-scale modelingko_KR
dc.subjectSystems biologyko_KR
dc.subjectMetabolic engineeringko_KR
dc.subjectYarrowia lipolyticako_KR
dc.subjecteMOMAko_KR
dc.subjectLipidko_KR
dc.subjectNon-conventional yeastko_KR
dc.subjectTAGko_KR
dc.titleIn silico identification of metabolic engineering strategies for improved lipid production in Yarrowia lipolytica by genome-scale metabolic modelingko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor김민석-
dc.contributor.AlternativeAuthor박범기-
dc.contributor.AlternativeAuthor김은정-
dc.contributor.AlternativeAuthor김준원-
dc.contributor.AlternativeAuthor김병기-
dc.identifier.doi10.1186/s13068-019-1518-4-
dc.language.rfc3066en-
dc.rights.holderThe Author(s)-
dc.date.updated2019-07-28T03:40:51Z-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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