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Efficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution

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dc.contributor.authorLee, Jong Kwan-
dc.contributor.authorKim, Sujin-
dc.contributor.authorKim, Wonsik-
dc.contributor.authorKim, Sungil-
dc.contributor.authorCha, Seungwoo-
dc.contributor.authorMoon, Hankyeol-
dc.contributor.authorHur, Dong Hoon-
dc.contributor.authorKim, Seon‑Young-
dc.contributor.authorNa, Jeong‑Geol-
dc.contributor.authorLee, Jin Won-
dc.contributor.authorLee, Eun Yeol-
dc.contributor.authorHahn, Ji‑Sook-
dc.date.accessioned2020-01-15T07:03:49Z-
dc.date.available2020-01-15T16:05:30Z-
dc.date.issued2019-09-30-
dc.identifier.citationBiotechnology for Biofuels, 12(1):234ko_KR
dc.identifier.issn1754-6834-
dc.identifier.uri10.1186/s13068-019-1574-9-
dc.identifier.urihttps://hdl.handle.net/10371/163020-
dc.description.abstractBackground
Methane, a main component of natural gas and biogas, has gained much attention as an abundant and low-cost carbon source. Methanotrophs, which can use methane as a sole carbon and energy source, are promising hosts to produce value-added chemicals from methane, but their metabolic engineering is still challenging. In previous attempts to produce lactic acid (LA) from methane, LA production levels were limited in part due to LA toxicity. We solved this problem by generating an LA-tolerant strain, which also contributes to understanding novel LA tolerance mechanisms.

Results
In this study, we engineered a methanotroph strain Methylomonas sp. DH-1 to produce d-lactic acid (d-LA) from methane. LA toxicity is one of the limiting factors for high-level production of LA. Therefore, we first performed adaptive laboratory evolution of Methylomonas sp. DH-1, generating an LA-tolerant strain JHM80. Genome sequencing of JHM80 revealed the causal gene watR, encoding a LysR-type transcription factor, whose overexpression due to a 2-bp (TT) deletion in the promoter region is partly responsible for the LA tolerance of JHM80. Overexpression of the watR gene in wild-type strain also led to an increase in LA tolerance. When d form-specific lactate dehydrogenase gene from Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 was introduced into the genome while deleting the glgA gene encoding glycogen synthase, JHM80 produced about 7.5-fold higher level of d-LA from methane than wild type, suggesting that LA tolerance is a critical limiting factor for LA production in this host. d-LA production was further enhanced by optimization of the medium, resulting in a titer of 1.19g/L and a yield of 0.245g/g CH4.

Conclusions
JHM80, an LA-tolerant strain of Methylomonas sp. DH-1, generated by adaptive laboratory evolution was effective in LA production from methane. Characterization of the mutated genes in JHM80 revealed that overexpression of the watR gene, encoding a LysR-type transcription factor, is responsible for LA tolerance. By introducing a heterologous lactate dehydrogenase gene into the genome of JHM80 strain while deleting the glgA gene, high d-LA production titer and yield were achieved from methane.
ko_KR
dc.description.sponsorshipThis work was supported by C1 Gas Refnery Program through the National Research Foundation of Korean (NRF) funded by the Ministry of Science and ICT (2016M3D3A01913245).ko_KR
dc.language.isoenko_KR
dc.publisherBMCko_KR
dc.subjectAdaptive laboratory evolution-
dc.subjectd-Lactate-
dc.subjectLysR-type transcriptional regulator-
dc.subjectMethane-
dc.subjectMethylomonas sp. DH-1-
dc.titleEfficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolutionko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor이종관-
dc.contributor.AlternativeAuthor김수진-
dc.contributor.AlternativeAuthor김원식-
dc.contributor.AlternativeAuthor김성일-
dc.contributor.AlternativeAuthor차승우-
dc.contributor.AlternativeAuthor문한결-
dc.contributor.AlternativeAuthor허동훈-
dc.contributor.AlternativeAuthor김선영-
dc.contributor.AlternativeAuthor이진원-
dc.contributor.AlternativeAuthor이은열-
dc.contributor.AlternativeAuthor한지숙-
dc.citation.journaltitleBiotechnology for Biofuelsko_KR
dc.language.rfc3066en-
dc.rights.holderThe Author(s)-
dc.date.updated2019-10-06T03:25:08Z-
dc.citation.number1ko_KR
dc.citation.startpage234ko_KR
dc.citation.volume12ko_KR
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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