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Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells
Cited 22 time in
Web of Science
Cited 23 time in Scopus
- Authors
- Issue Date
- 2006-01
- Publisher
- Kluwer Academic Publishers
- Citation
- Biotechnology Letters, Vol.28 No.1, pp.9-15
- Abstract
- A randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production similar to 10-fold at similar to 60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells.
- ISSN
- 0141-5492
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