Publications

Detailed Information

Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells

Cited 22 time in Web of Science Cited 23 time in Scopus
Authors

Kwon, Ryuk-Jun; Kim, Seong Keun; Lee, Seung-Il; Hwang, Su-Jeong; Lee, Gyun Min; Kim, Jin-Soo; Seol, Wongi

Issue Date
2006-01
Publisher
Kluwer Academic Publishers
Citation
Biotechnology Letters, Vol.28 No.1, pp.9-15
Abstract
A randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production similar to 10-fold at similar to 60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells.
ISSN
0141-5492
URI
https://hdl.handle.net/10371/165607
DOI
https://doi.org/10.1007/s10529-005-4680-7
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Related Researcher

  • College of Natural Sciences
  • Department of Chemistry
Research Area Biology and Biochemistry

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share