Knockout of the ribonuclease inhibitor gene leaves human cells vulnerable to secretory ribonucleases
- Thomas, Sydney P.; Kim, Eunji; Kim, Jin-Soo; Raines, Ronald T.
- Issue Date
- Biochemistry, Vol.55 No.46, pp.6359-6362
- Ribonuclease inhibitor (RNH1) is a cytosolic protein that binds with femtomolar affinity to human ribonuclease 1 (RNase 1) and homologous secretory ribonucleases. RNH1 contains 32 cysteine residues and has been implicated as an antioxidant. Here, we use CRISPR-Cas9 to knock out RNH1 in HeLa cells. We find that cellular RNH1 affords marked protection from the lethal ribonucleolytic activity of RNase 1 but not from oxidants. We conclude that RNH1 protects cytosolic RNA from invading ribonucleases.
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