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Directed evolution of CRISPR-Cas9 to increase its specificity
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jungjoon K. | - |
dc.contributor.author | Jeong, Euihwan | - |
dc.contributor.author | Lee, Joonsun | - |
dc.contributor.author | Jung, Minhee | - |
dc.contributor.author | Shin, Eunji | - |
dc.contributor.author | Kim, Young-hoon | - |
dc.contributor.author | Lee, Kangin | - |
dc.contributor.author | Jung, Inyoung | - |
dc.contributor.author | Kim, Daesik | - |
dc.contributor.author | Kim, Seokjoong | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.date.accessioned | 2020-04-27T12:57:48Z | - |
dc.date.available | 2020-04-27T12:57:48Z | - |
dc.date.created | 2019-08-02 | - |
dc.date.created | 2019-08-02 | - |
dc.date.issued | 2018-08-06 | - |
dc.identifier.citation | Nature Communications, Vol.9, p. 3048 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.other | 80405 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165696 | - |
dc.description.abstract | The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects. Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher specificities than the wild-type SpCas9 protein are available, these attenuated Cas9 variants are often poorly efficient in human cells. Here, we develop a directed evolution approach in E. coli to obtain Sniper-Cas9, which shows high specificities without killing on-target activities in human cells. Unlike other engineered Cas9 variants, Sniper-Cas9 shows WT-level on-target activities with extended or truncated sgRNAs with further reduced off-target activities and works well in a preassembled ribonucleoprotein (RNP) format to allow DNA-free genome editing. | - |
dc.language | 영어 | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Directed evolution of CRISPR-Cas9 to increase its specificity | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1038/s41467-018-05477-x | - |
dc.citation.journaltitle | Nature Communications | - |
dc.identifier.wosid | 000440776800001 | - |
dc.identifier.scopusid | 2-s2.0-85051259374 | - |
dc.citation.startpage | 3048 | - |
dc.citation.volume | 9 | - |
dc.identifier.sci | 000440776800001 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | WIDE TARGET SPECIFICITIES | - |
dc.subject.keywordPlus | HUMAN HEMATOPOIETIC STEM | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | CAS9 | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | NUCLEASES | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | GUIDE | - |
dc.subject.keywordPlus | ENDONUCLEASES | - |
dc.subject.keywordPlus | GENERATION | - |
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