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Nanoscale enzyme reactors in mesoporous carbon for improved performance and lifetime of biosensors and biofuel cells

Cited 41 time in Web of Science Cited 43 time in Scopus
Authors
Kwon, Ki Young; Youn, Jongkyu; Kim, Jae Hyun; Park, Yongjin; Jeon, Chulmin; Kim, Byoung Chan; Kwon, Yongchai; Zhao, Xueyan; Wang, Ping; Sang, Byoung In; Lee, Jinwoo; Park, Hyun Gyu; Chang, Ho Nam; Hyeon, Taeghwan; Ha, Su; Jung, Hee-Tae; Kim, Jungbae
Issue Date
2010-10
Citation
Biosensors and Bioelectronics, Vol.26 No.2, pp.655-660
Keywords
Biofuel cellsBiosensorsEnzyme crosslinkingMesoporous carbonNanoscale enzyme reactors
Abstract
Nanoscale enzyme reactors (NERs) of glucose oxidase in conductive mesoporous carbons were prepared in a two-step process of enzyme adsorption and follow-up enzyme crosslinking. MSU-F-C, a mesoprous carbon, has a bottleneck pore structure with mesocellular pores of 26 nm connected with window mesopores of 17 nm. This structure enables the ship-in-a-bottle mechanism of NERs, which effectively prevents the crosslinked enzymes in mesocellular pores from leaching through the smaller window mesopores. This NER approach not only stabilized the enzyme but also expedited electron transfer between the enzyme and the conductive MSU-F-C by maintaining a short distance between them. In a comparative study with GOx that was simply adsorbed without crosslinking, the NER approach was proven to be effective in improving the sensitivity of glucose biosensors and the power density of biofuel cells. The power density of biofuel cells could be further improved by manipulating several factors, such as by adding a mediator, changing the order of adsorption and crosslinking, and inserting a gold mesh as an electron collector. (C) 2010 Elsevier B.V. All rights reserved.
ISSN
0956-5663
URI
https://hdl.handle.net/10371/165788
DOI
https://doi.org/10.1016/j.bios.2010.07.001
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Chemical Convergence for Energy and Environment (에너지환경 화학융합기술전공)Journal Papers (저널논문_에너지환경 화학융합기술전공)
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