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High performance immunoassay using immobilized enzyme in nanoporous carbon
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Piao, Yunxian | - |
dc.contributor.author | Lee, Dohoon | - |
dc.contributor.author | Kim, Jungbae | - |
dc.contributor.author | Kim, Jaeyun | - |
dc.contributor.author | Hyeon, Taeghwan | - |
dc.contributor.author | Kim, Hak-Sung | - |
dc.date.accessioned | 2020-04-27T13:21:09Z | - |
dc.date.available | 2020-04-27T13:21:09Z | - |
dc.date.created | 2020-03-18 | - |
dc.date.issued | 2009-05 | - |
dc.identifier.citation | The Analyst, Vol.134 No.5, pp.926-932 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.other | 92812 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165793 | - |
dc.description.abstract | A highly stable immunoassay format was constructed using signal-generating enzyme immobilized in nanoporous carbon. A mesocellular carbon foam, called MSU-F-C, was loaded with horseradish peroxidase (HRP), followed by cross-linking of the enzyme using glutaraldehyde (GA) and modification of the surface with anti-human IgG through EDC/sulfo-NHS chemistry. The resulting MSU-F-C/HRP/anti-human IgG stably retained immobilized enzymes and antibodies, showing higher thermal stability. The MSU-F-C/HRP/anti-human IgG retained about 80% of initial enzyme activity at 40 degrees C after a 5 h incubation, while the HRP/anti-human IgG conjugate resulted in almost 90% loss of initial activity in the same condition. In bead-based immunoassays, the signal amplification using MSU-F-C/HRP/anti-human IgG enabled the sensitive colorimetric detection of a target analyte, human IgG, in a detection limit of similar to 33 pM, with negligible cross-reactivity against rabbit and chicken IgGs. | - |
dc.language | 영어 | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | High performance immunoassay using immobilized enzyme in nanoporous carbon | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 현택환 | - |
dc.identifier.doi | 10.1039/b813451k | - |
dc.citation.journaltitle | The Analyst | - |
dc.identifier.wosid | 000265355600017 | - |
dc.identifier.scopusid | 2-s2.0-65349117038 | - |
dc.citation.endpage | 932 | - |
dc.citation.number | 5 | - |
dc.citation.startpage | 926 | - |
dc.citation.volume | 134 | - |
dc.identifier.sci | 000265355600017 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Hyeon, Taeghwan | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | BIOLOGICAL WARFARE AGENTS | - |
dc.subject.keywordPlus | CATALYTIC ACTIVITY | - |
dc.subject.keywordPlus | MESOPOROUS SILICA | - |
dc.subject.keywordPlus | NANOTUBES | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordPlus | COMPOSITE | - |
dc.subject.keywordPlus | PROTEINS | - |
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