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College of Engineering/Engineering Practice School (공과대학/대학원)
Dept. of Chemical and Biological Engineering (화학생물공학부)
Journal Papers (저널논문_화학생물공학부)
MR tracking of transplanted cells with "positive contrast" using manganese oxide nanoparticles
- Issue Date
- 2008-07
- Publisher
- John Wiley & Sons Inc.
- Citation
- Magnetic Resonance in Medicine, Vol.60 No.1, pp.1-7
- Abstract
- Rat glioma cells were labeled using electroporation with either manganese oxide (MnO) or superparamagnetic iron oxide (SPIO) nanoparticles. The viability and proliferation of SPIO-labeled cells (1.9 mg Fe/ml) or cells electroporated with a low dose of MnO (100 mu g Mn/ml) was not significantly different from unlabeled cells; a higher MnO dose (785 mu g Mn/ml) was found to be toxic. The cellular ion content was 0.1-0.3 pg Mn/cell and 4.4 pg Fe/cell, respectively, with cellular relaxivities of 2.5-4.8 s(-1) (RI) and 45-84 s(-1) (R-2) for MnO-labeled cells. Labeled cells (SPIO and low-dose MnO) were each transplanted in contralateral brain hemispheres of rats and imaged in vivo at 9.4T. While SPIO-labeled cells produced a strong "negative contrast" due to the increase in R-2, MnO-Iabeled cells produced "positive contrast" with an increased R-1. Simultaneous imaging of both transplants with opposite contrast offers a method for MR "double labeling" of different cell populations.
- ISSN
- 0740-3194
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