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Single enzyme nanoparticles armored by a thin silicate network: Single enzyme caged nanoparticles

Cited 11 time in Web of Science Cited 11 time in Scopus
Authors
Hong, Sung-Gil; Kim, Byoung Chan; Bin Na, Hyon; Lee, Jinwoo; Youn, Jongkyu; Chung, Seung-Wook; Lee, Chang-Won; Lee, Byoungsoo; Kim, Han Sol; Hsiao, Erik; Kim, Seong H.; Kim, Byung-Gee; Park, Hyun Gyu; Chang, Ho Nam; Hyeon, Taeghwan; Dordick, Jonathan S.; Grate, Jay W.; Kim, Jungbae
Issue Date
2017-08
Citation
Chemical Engineering Journal, Vol.322, pp.510-515
Keywords
EnzymesEnzyme catalysisSingle enzyme caged nanoparticlesEnzyme stabilizationEnzyme immobilization
Abstract
For the encapsulation of biomolecules in inorganic materials, we have developed a unique enzyme silicate conjugate material that consists of a self-assembled molecularly thin silicate layer on the surface of each individual enzyme molecule. The enzyme-silicate conjugate materials, called single enzyme caged nanoparticles (SECNs), were synthesized via the silica polymerization on the surface of enzyme molecule after solubilizing each enzyme molecule in hexane by using a tiny amount of surfactant, called "ion-pairing". SECNs possess near native enzyme activity in aqueous media with minimal substrate diffusional limitations, and are highly stable under the protection of silicate network cage. Due to their nearly molecular size, SECNs can also be adsorbed into mesoporous silica materials to yield robust and easily-recyclable enzymatic systems that can be used in a number of potential biocatalytic applications such as diagnostics, biosensors, biotransformations, biofuel production, bioremediation and CO2 capture.
ISSN
1385-8947
URI
https://hdl.handle.net/10371/165948
DOI
https://doi.org/10.1016/j.cej.2017.04.022
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Chemical Convergence for Energy and Environment (에너지환경 화학융합기술전공)Journal Papers (저널논문_에너지환경 화학융합기술전공)
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