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Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins
Cited 419 time in
Web of Science
Cited 435 time in Scopus
- Authors
- Issue Date
- 2006-08
- Publisher
- American Chemical Society
- Citation
- Journal of the American Chemical Society, Vol.128 No.33, pp.10658-10659
- Abstract
- Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads. Copyright © 2006 American Chemical Society.
- ISSN
- 0002-7863
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