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Simplified EM grid vitrification is a convenient and efficient method for mouse mature oocyte cryopreservation

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Authors
Kim, Seok Hyun; Ku, Seung-Yup; Sung, Ki Cheong; Kang, Moon Joo; Kim, Sung Ah; Kim, Hee Sun; Oh, Sun Kyung; Jee, Byung Chul; Suh, Chang Suk; Choi, Young Min; Kim, Jung Gu; Moon, Shin Yong
Issue Date
2006-06-30
Publisher
Yonsei University College of Medicine
Citation
Yonsei Med J. 2006 Jun 30;47(3):399-404.
Keywords
AnimalsCell SurvivalCryopreservation/*instrumentation/*methodsFemale*Fertilization in VitroMaleMiceMice, Inbred C57BLMice, Inbred DBAOocytes/*cytologyPregnancy
Abstract
This study was performed to evaluate the efficiency of simplified EM grid vitrification, skipping the step of removing the cryoprotectant (5.5M EG + 1.0M sucrose) droplet on the grid after loading oocytes, compared to conventional cryopreservation protocols for mouse mature oocytes. Firstly, the recovery, survival, fertilization and hatching rates of simplified EM grid vitrification were compared with those of the slow freezing method using 1.5M DMSO. Then, conventional EM grid vitrification was compared with simplified EM grid vitrification. Simplified EM grid vitrification showed higher survival, fertilization and hatching rates than those of the slow freezing method (85.6% vs. 63.2%; 51.0% vs. 22.3%; 38.7% vs. 12.5%, p < 0.01, respectively). Moreover, simplified EM grid vitrification showed higher recovery, survival and fertilization rates than those of conventional EM grid vitrification (100% vs. 95.0%, p=0.024; 90.0% vs. 78.9%, p=0.033; 56.7% vs. 38.7%, p=0.021, respectively). Hatching rate tended to be higher for simplified EM grid vitrification compared to conventional EM grid vitrification (41.1% vs. 24.1%). In conclusion, simplified EM grid vitrification is a convenient and efficient method for cryopreservation of mouse mature oocytes, compared to conventional EM grid vitrification and slow freezing methods.
ISSN
0513-5796 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16807991

https://hdl.handle.net/10371/16597
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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