Browse

Diversity of bacterial community in freshwater of Woopo wetland

Cited 22 time in Web of Science Cited 24 time in Scopus
Authors
Baik, Keun Sik; Park, Seong Chan; Kim, Eun Mi; Bae, Kyung Sook; Ann, Jae-Hyung; Ka, Jong-Ok; Chun, Jongsik; Seong, Chi Nam
Issue Date
2008-12
Citation
The Journal of Microbiology, Vol.46 No.6, pp.647-655
Keywords
diversityWoopo wetlandbacterial isolates16S rRNA gene clonesphylotypes
Abstract
Diversity of bacterial community in water layer of Woopo wetland was investigated. Cultivable bacterial strains were isolated by the standard dilution plating technique and culture-independent 16S rRNA gene clones were obtained directly from DNA extracts of a water sample. Amplified rDNA restriction analysis (ARDRA) was applied onto both of the isolates and 16S rRNA gene clones. Rarefaction curves, coverage rate and diversity indices of ARDRA patterns were calculated. Representative isolates and clones of all the single isolate/clone phylotype were partially sequenced and analyzed phylogenetically. Sixty-four and 125 phylotypes were obtained from 203 bacterial isolates and 235 culture-independent 16S rRNA gene clones, respectively. Bacterial isolates were composed of 4 phyla, of which Firmicutes (49.8%) and Actinobacteria (32.0%) were predominant. Isolates were affiliated with 58 species. Culture-independent 16S rRNA gene clones were composed of 8 phyla, of which Proteobacteria (62.2%), Actinobacteria (15.5%), and Bacteroidetes (13.7%) were predominant. Diversity of 16S rRNA gene clones originated from cultivation-independent DNA extracts was higher than that of isolated bacteria.
ISSN
1225-8873
URI
https://hdl.handle.net/10371/165983
DOI
https://doi.org/10.1007/s12275-008-0135-x
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse