S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Chemical and Biological Engineering (화학생물공학부) Journal Papers (저널논문_화학생물공학부)
A magnetically separable, highly stable enzyme system based on nanocomposites of enzymes and magnetic nanoparticles shipped in hierarchically ordered, mesocellular, mesoporous silica
- Kim, Jungbae; Lee, Jinwoo; Na, Hyon Bin; Kim, Byoung Chan; Youn, Jong Kyu; Kwak, Ja Hun; Moon, Karam; Lee, Eunwoong; Kim, Jaeyun; Park, Jongnam; Dohnalkova, Alice; Park, Hyun Gyu; Gu, Man Bock; Chang, Ho Nam; Grate, Jay W.; Hyeon, Taeghwan
- Issue Date
- SMALL, Vol.1 No.12, pp.1203-1207
- enzyme catalysis; magnetic nanoparticles; magnetic separation; mesoporous materials; silica
- The development of a magnetically separable and highly stable enzyme system, using nanoparticles and mesoporous silica, was investigated. Nanometer-scale composites of enzyme molecules and hierarchically ordered magnetite nanoparticles were immobilized via a ship-in-a-bottle approach, which used co-adsorption of enzymes and magnetite (Fe3O4) nanoparticles. The enzyme molecules were crosslinked via glutaraldehyde (GA) treatment resulting in the effective entrapment of neighboring magnetite nanoparticles. These nanocomposites, called M-CLEAs, were found magnetically separable, highly loaded with enzymes, stable under harsh conditions, resistant to proteolytic digestion, and recyclable for iterative use with negligible loss of enzyme activity. The ship-in-a-bottle approach can be expanded to many other enzymes and has applications in bioremediation and bioconversion.
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- College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Chemical Convergence for Energy and Environment (에너지환경 화학융합기술전공)Journal Papers (저널논문_에너지환경 화학융합기술전공)