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A magnetically separable, highly stable enzyme system based on nanocomposites of enzymes and magnetic nanoparticles shipped in hierarchically ordered, mesocellular, mesoporous silica

Cited 100 time in Web of Science Cited 104 time in Scopus
Authors
Kim, Jungbae; Lee, Jinwoo; Na, Hyon Bin; Kim, Byoung Chan; Youn, Jong Kyu; Kwak, Ja Hun; Moon, Karam; Lee, Eunwoong; Kim, Jaeyun; Park, Jongnam; Dohnalkova, Alice; Park, Hyun Gyu; Gu, Man Bock; Chang, Ho Nam; Grate, Jay W.; Hyeon, Taeghwan
Issue Date
2005-12
Citation
SMALL, Vol.1 No.12, pp.1203-1207
Keywords
enzyme catalysismagnetic nanoparticlesmagnetic separationmesoporous materialssilica
Abstract
The development of a magnetically separable and highly stable enzyme system, using nanoparticles and mesoporous silica, was investigated. Nanometer-scale composites of enzyme molecules and hierarchically ordered magnetite nanoparticles were immobilized via a ship-in-a-bottle approach, which used co-adsorption of enzymes and magnetite (Fe3O4) nanoparticles. The enzyme molecules were crosslinked via glutaraldehyde (GA) treatment resulting in the effective entrapment of neighboring magnetite nanoparticles. These nanocomposites, called M-CLEAs, were found magnetically separable, highly loaded with enzymes, stable under harsh conditions, resistant to proteolytic digestion, and recyclable for iterative use with negligible loss of enzyme activity. The ship-in-a-bottle approach can be expanded to many other enzymes and has applications in bioremediation and bioconversion.
ISSN
1613-6810
URI
https://hdl.handle.net/10371/165998
DOI
https://doi.org/10.1002/smll.200500245
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Chemical Convergence for Energy and Environment (에너지환경 화학융합기술전공)Journal Papers (저널논문_에너지환경 화학융합기술전공)
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