Publications
Detailed Information
Multi-modal transfection agent based on monodisperse magnetic. nanoparticles for stem cell gene delivery and tracking
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Wooram | - |
dc.contributor.author | Yang, Han Na | - |
dc.contributor.author | Ling, Daishun | - |
dc.contributor.author | Yim, Hyeona | - |
dc.contributor.author | Kim, Kyoung Sub | - |
dc.contributor.author | Hyeon, Taeghwan | - |
dc.contributor.author | Na, Kun | - |
dc.contributor.author | Park, Keun-Hong | - |
dc.date.accessioned | 2020-04-27T13:44:19Z | - |
dc.date.available | 2020-04-27T13:44:19Z | - |
dc.date.created | 2018-01-10 | - |
dc.date.issued | 2014-08 | - |
dc.identifier.citation | Biomaterials, Vol.35 No.25, pp.7239-7247 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.other | 11831 | - |
dc.identifier.uri | https://hdl.handle.net/10371/166098 | - |
dc.description.abstract | Directing the controlled differentiation and tracking of stem cells is essential to achieve successful stem cell therapy. In this work, we describe a multi-modal (MR/optical) transfection agent (MTA) for efficient gene delivery and cell tracking of human mesenchymal stem cells (hMSCs). The MTA was synthesized through a facile two-step approach with 1) ligand exchange of a catechol-functionalized polypeptide (CFP) and 2) chemical immobilization of fluorescence labelled cationic polymer via aminolysis reaction. Cationic polymer-immobilized MIAs with size of 40 nm exhibit greatly enhanced colloidal stability in aqueous solution. In addition, the MTAs were capable of binding DNA molecules for transfection. The MTA/pDNA complex showed relatively good transfection efficiency in hMSCs (compared to the commercial transfection agent, Lipofectamine) and good biocompatibility. MTA-treated hMSCs were successfully visualized after transplantation via MR and optical imaging system over 14 days. These studies highlight the challenges associated with the potential advantages of designing multi-modal nanostructured materials as tools for genetic materials delivery and cell-tracking in stem cell therapy. (C) 2014 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Pergamon Press Ltd. | - |
dc.title | Multi-modal transfection agent based on monodisperse magnetic. nanoparticles for stem cell gene delivery and tracking | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 현택환 | - |
dc.identifier.doi | 10.1016/j.biomaterials.2014.05.010 | - |
dc.citation.journaltitle | Biomaterials | - |
dc.identifier.wosid | 000338386800054 | - |
dc.identifier.scopusid | 2-s2.0-84902085523 | - |
dc.citation.endpage | 7247 | - |
dc.citation.number | 25 | - |
dc.citation.startpage | 7239 | - |
dc.citation.volume | 35 | - |
dc.identifier.sci | 000338386800054 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Hyeon, Taeghwan | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | IRON-OXIDE NANOPARTICLES | - |
dc.subject.keywordPlus | INORGANIC NANOPARTICLES | - |
dc.subject.keywordPlus | BIOMEDICAL APPLICATIONS | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | PHOTOSENSITIZER | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | INTERNALIZATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordAuthor | Multimodal imaging | - |
dc.subject.keywordAuthor | Magnetic resonance imaging | - |
dc.subject.keywordAuthor | Cell tracking | - |
dc.subject.keywordAuthor | Stem cells | - |
dc.subject.keywordAuthor | Catechol | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.