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Characterization of splenic MRC1hiMHCIIlo and MRC1loMHCIIhi cells from the monocyte/macrophage lineage of White Leghorn chickens
DC Field | Value | Language |
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dc.contributor.author | Yu, Keesun | - |
dc.contributor.author | Gu, Min Jeong | - |
dc.contributor.author | Pyung, Young Jin | - |
dc.contributor.author | Song, Ki-Duk | - |
dc.contributor.author | Park, Tae Sub | - |
dc.contributor.author | Han, Seung Hyun | - |
dc.contributor.author | Yun, Cheol-Heui | - |
dc.date.accessioned | 2020-07-20T06:10:05Z | - |
dc.date.available | 2020-07-20T15:11:44Z | - |
dc.date.issued | 2020-05-27 | - |
dc.identifier.citation | Veterinary Research. 2020 May 27;51(1):73 | ko_KR |
dc.identifier.issn | 1297-9716 | - |
dc.identifier.uri | https://hdl.handle.net/10371/168616 | - |
dc.description.abstract | Monocytes/macrophages, which are found in a variety of organs, maintain tissue homeostasis at a steady state and act as the first line of defence during pathogen-induced inflammation in the host. Most monocyte/macrophage lineage studies in chickens have been largely performed using cell lines, while few studies using primary cellshave been conducted. In the present study, the phenotypic and functional characteristics of splenic monocyte/macrophage lineage cells during steady state and inflammatory conditions were examined. Splenic monocyte/macrophage lineage cells could be identified as MRC1loMHCIIhi and MRC1hiMHCIIlo cells based on their surface expression of MRC1 and MHCII. In the steady state, MRC1loMHCIIhi cells were more frequently found among MRC1+ cells. MRC1loMHCIIhi cells expressed a higher number of antigen-presenting molecules (MHCII, MHCI, and CD80) than MRC1hiMHCIIlo cells. In contrast, MRC1hiMHCIIlo cells showed better phagocytic and CCR5-dependent migratory properties than MRC1loMHCIIhi cells. Furthermore, MRC1hiMHCIIlo cells infiltrated the spleen in vivo and then became MRC1loMHCIIhi cells. During lipopolysaccharide (LPS)-induced inflammatory conditions that were produced via intraperitoneal (i.p.) injection, the proportion and absolute number of MRC1hiMHCIIlo cells were increased in the spleen. Uniquely, inflammation induced the downregulation of MHCII expression in MRC1hiMHCIIlo cells. The major source of inflammatory cytokines (IL-1β, IL-6, and IL-12) was MRC1loMHCIIhi cells. Furthermore, MRC1hiMHCIIlo cells showed greater bactericidal activity than MRC1loMHCIIhi cells during LPS-induced inflammation. Collectively, these results suggest that two subsets of monocyte/macrophage lineage cells exist in the chicken spleen that have functional differences. | ko_KR |
dc.description.sponsorship | This research was performed with funding from the National Research Foun‑dation (NRF-2018R1A2B2006793), Republic of Korea. This research was also supported by the Cooperative Research Programme for Agriculture Science and Technology Development (Project No. PJ01336401), Rural Development Administration, Republic of Korea. KSY, MJG and YJP were supported by the
BK21 Plus Programme of the Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.title | Characterization of splenic MRC1hiMHCIIlo and MRC1loMHCIIhi cells from the monocyte/macrophage lineage of White Leghorn chickens | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 유기선 | - |
dc.contributor.AlternativeAuthor | 구민정 | - |
dc.contributor.AlternativeAuthor | 평영진 | - |
dc.contributor.AlternativeAuthor | 송기덕 | - |
dc.contributor.AlternativeAuthor | 박태섭 | - |
dc.contributor.AlternativeAuthor | 한승현 | - |
dc.contributor.AlternativeAuthor | 윤철희 | - |
dc.identifier.doi | 10.1186/s13567-020-00795-9 | - |
dc.citation.journaltitle | Veterinary Research | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2020-06-16T09:40:34Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 73 | ko_KR |
dc.citation.volume | 51 | ko_KR |
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