Browse

Adenine base editors catalyze cytosine conversions in human cells

Cited 26 time in Web of Science Cited 27 time in Scopus
Authors
Kim, Heon Seok; Jeong, You Kyeong; Hur, Junho K.; Kim, Jin-Soo; Bae, Sangsu
Issue Date
2019-10
Citation
Nature Biotechnology, Vol.37 No.10, pp.1145-1148
Abstract
Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5-7) and in a confined TC*N sequence context. Adenine base editor-induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing.
ISSN
1087-0156
URI
https://hdl.handle.net/10371/171743
DOI
https://doi.org/10.1038/s41587-019-0254-4
Files in This Item:
There are no files associated with this item.
Appears in Collections:
Seoul National University(서울대학교)Featured Researcher's Articles
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse