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TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms

Cited 55 time in Web of Science Cited 60 time in Scopus
Authors
Kim, Boseon; Ha, Minju; Loeff, Luuk; Chang, Hyeshik; Simanshu, Dhirendra K.; Li, Sisi; Fareh, Mohamed; Patel, Dinshaw J.; Joo, Chirlmin; Kim, V. Narry
Issue Date
2015-07
Citation
EMBO Journal, Vol.34 No.13, pp.1801-1815
Keywords
precursor microRNAsingle-molecule fluorescenceTUT4 (ZCCHC11)TUT7 (ZCCHC6)uridylation
Abstract
Terminal uridylyl transferases (TUTs) function as integral regulators of microRNA (miRNA) biogenesis. Using biochemistry, single-molecule, and deep sequencing techniques, we here investigate the mechanism by which human TUT7 (also known as ZCCHC6) recognizes and uridylates precursor miRNAs (pre-miRNAs) in the absence of Lin28. We find that the overhang of a pre-miRNA is the key structural element that is recognized by TUT7 and its paralogues, TUT4 (ZCCHC11) and TUT2 (GLD2/PAPD4). For group II pre-miRNAs, which have a 1-nt 3' overhang, TUT7 restores the canonical end structure (2-nt 3' overhang) through mono-uridylation,thereby promoting miRNA biogenesis. For pre-miRNAs where the 3' end is further recessed into the stem (as in 3' trimmed pre-miRNAs), TUT7 generates an oligo-U tail that leads to degradation. In contrast to Lin28-stimulated oligo-uridylation, which is processive, a distributive mode is employed by TUT7 for both mono- and oligo-uridylation in the absence of Lin28. The overhang length dictates the frequency (but not duration) of the TUT7-RNA interaction, thus explaining how TUT7 differentiates pre-miRNA species with different overhangs. Our study reveals dual roles and mechanisms of uridylation in repair and removal of defective pre-miRNAs.
ISSN
0261-4189
URI
https://hdl.handle.net/10371/171888
DOI
https://doi.org/10.15252/embj.201590931
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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