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Regulation of poly(A) tail and translation during the somatic cell cycle

Cited 73 time in Web of Science Cited 73 time in Scopus
Authors
Park, Jong-Eun; Yi, Hyerim; Kim, Yoosik; Chang, Hyeshik; Kim, V. Narry
Issue Date
2016-05
Citation
Molecular Cell, Vol.62 No.3, pp.462-471
Abstract
Poly(A) tails are critical for mRNA stability and translation. However, recent studies have challenged this view, showing that poly(A) tail length and translation efficiency are decoupled in non-embryonic cells. Using TAIL-seq and ribosome profiling, we investigate poly(A) tail dynamics and translational control in the somatic cell cycle. We find dramatic changes in poly(A) tail lengths of cell-cycle regulatory genes like CDK1, TOP2A, and FBXO5, explaining their translational repression in M phase. We also find that poly(A) tail length is coupled to translation when the poly(A) tail is <20 nucleotides. However, as most genes have >20 nucleotide poly(A) tails, their translation is regulated mainly via poly(A) tail length-independent mechanisms during the cell cycle. Specifically, we find that terminal oligopyrimidine (TOP) tract-containing transcripts escape global translational suppression in M phase and are actively translated. Our quantitative and comprehensive data provide a revised view of translational control in the somatic cell cycle.
ISSN
1097-2765
URI
https://hdl.handle.net/10371/171960
DOI
https://doi.org/10.1016/j.molcel.2016.04.007
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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