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Two splice variants of the IDD14 transcription factor competitively form nonfunctional heterodimers which may regulate starch metabolism

Cited 97 time in Web of Science Cited 100 time in Scopus
Authors
Seo, Pil Joon; Kim, Mi Jung; Ryu, Jae-Yong; Jeong, Eun-Young; Park, Chung-Mo
Issue Date
2011-05
Citation
Nature Communications, Vol.2
Abstract
Alternative splicing of primary gene transcripts provides eukaryotic cells, with a critical scheme for enriching transcriptome and proteome diversity. Here we report that alternative splicing of the Arabidopsis INDERMINATE DOMAIN 14 (IDD14) transcription factor gene generates a competitive inhibitor in regulating starch metabolism. An alternatively spliced IDD14 form (IDD14 beta), which is produced predominantly under cold conditions, lacks functional DNA-binding domain but is able to form heterodimers with the functional IDD14 form (IDD14 alpha). IDD14 alpha-IDD14 beta heterodimers have reduced binding activity to the promoter of Qua-Quine Starch (QQS) gene that regulates starch accumulation. Transgenic Arabidopsis plants overproducing IDD14 alpha (35S:IDD14 alpha) exhibited retarded growth with pale green leaves as appeared on QQS-overexpressing plants. Notably, IDD14 beta overproduction rescued the 35S:IDD14 alpha phenotypes. We propose that alternative splicing of the IDD14 gene generates a self-controlled regulatory loop that may modulate starch accumulation in response to cold.
ISSN
2041-1723
URI
https://hdl.handle.net/10371/172019
DOI
https://doi.org/10.1038/ncomms1303
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College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
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