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Covalent conjugation of mechanically stiff graphene oxide flakes to three-dimensional collagen scaffolds for osteogenic differentiation of human mesenchymal stem cells

Cited 75 time in Web of Science Cited 76 time in Scopus
Authors
Kang, Seokyung; Park, Jong Bo; Lee, Tae-Jin; Ryu, Seungmi; Bhang, Suk Ho; La, Wan-Geun; Noh, Myung-Kyung; Hong, Byung Hee; Kim, Byung-Soo
Issue Date
2015-03
Citation
Carbon, Vol.83, pp.162-172
Abstract
Mesenchymal stem cells (MSCs) preferentially differentiate to osteogenic lineage when cultured on mechanically stiff substrates. However, collagen sponges, clinically approved scaffolds for bone regeneration, provide soft microenvironment to MSCs. Here, we demonstrate that the covalent conjugation of mechanically stiff graphene oxide (GO) flakes to three-dimensional (3D) collagen scaffolds improves the mechanical properties of the scaffolds and promotes the osteogenic differentiation of human MSCs (hMSCs) cultured on the scaffolds. The covalent conjugation of GO flakes to collagen scaffolds increased the scaffold stiffness by 3-fold and did not cause cytotoxicity. hMSCs cultured on the GO-collagen scaffolds demonstrated significantly enhanced osteogenic differentiation compared to cells cultured on non-modified collagen scaffolds. The enhanced osteogenic differentiation observed on the stiffer scaffolds was likely mediated by MSC mechanosensing because molecules that are involved in cell adhesion to stiff substrates were either up-regulated or activated. The 3D GO-collagen scaffolds could offer a powerful platform for stem cell research and orthopedic regenerative medicine. (C) 2014 Elsevier Ltd. All rights reserved.
ISSN
0008-6223
URI
https://hdl.handle.net/10371/172174
DOI
https://doi.org/10.1016/j.carbon.2014.11.029
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College of Natural Sciences (자연과학대학)Dept. of Chemistry (화학부)Journal Papers (저널논문_화학부)
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