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Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy
DC Field | Value | Language |
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dc.contributor.author | Jun, Bong-Hyun | - |
dc.contributor.author | Noh, Mi Suk | - |
dc.contributor.author | Kim, Gunsung | - |
dc.contributor.author | Kang, Homan | - |
dc.contributor.author | Kim, Jong-Ho | - |
dc.contributor.author | Chung, Woo-Jae | - |
dc.contributor.author | Kim, Min-Soo | - |
dc.contributor.author | Kim, Yong-Kweon | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.contributor.author | Jeong, Dae Hong | - |
dc.contributor.author | Lee, Yoon-Sik | - |
dc.date.accessioned | 2021-01-31T08:37:26Z | - |
dc.date.available | 2021-01-31T08:37:26Z | - |
dc.date.created | 2020-12-15 | - |
dc.date.issued | 2009-08 | - |
dc.identifier.citation | Analytical Biochemistry, Vol.391 No.1, pp.24-30 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.other | 119192 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172294 | - |
dc.description.abstract | This article presents a prototype of a surface-enhanced Raman spectroscopy (SERS)-encoded magnetic bead of 8 mu m diameter. The core part of the bead is composed of a magnetic nanoparticle (NP)-embedded sulfonated polystyrene bead. The outer part of the bead is embedded with Ag NPs on which labeling molecules generating specific SERS bands are adsorbed. A silica shell is fabricated for further bioconjugation and protection of SERS signaling. Benzenethiol, 4-mercaptotoluene, 2-naphthalenethiol, and 4-aminothiophenol are used as labeling molecules. The magnetic SERS beads are used as Substrates for protein sensing and screening with easy handling. As a model application, streptavidin-bound magnetic SERS beads are used to illustrate selective separation in a flow cytometry system, and the screened beads are spectrally recognized by Raman spectroscopy. The proposed magnetic SERS beads are likely to be used as a versatile solid support for protein sensing and screening in multiple assay technology. (C) 2009 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Academic Press | - |
dc.title | Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.1016/j.ab.2009.05.005 | - |
dc.citation.journaltitle | Analytical Biochemistry | - |
dc.identifier.wosid | 000267063200004 | - |
dc.identifier.scopusid | 2-s2.0-67349278535 | - |
dc.citation.endpage | 30 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 24 | - |
dc.citation.volume | 391 | - |
dc.identifier.sci | 000267063200004 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Yong-Kweon | - |
dc.contributor.affiliatedAuthor | Cho, Myung-Haing | - |
dc.contributor.affiliatedAuthor | Jeong, Dae Hong | - |
dc.contributor.affiliatedAuthor | Lee, Yoon-Sik | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | FLOW-CYTOMETRY | - |
dc.subject.keywordPlus | MULTIPLEX IMMUNOASSAY | - |
dc.subject.keywordPlus | QUANTUM DOTS | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | SCATTERING | - |
dc.subject.keywordPlus | POLYSTYRENE | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordPlus | BIOMOLECULES | - |
dc.subject.keywordPlus | NANOPROBES | - |
dc.subject.keywordAuthor | Silver nanoparticles | - |
dc.subject.keywordAuthor | Optically active materials | - |
dc.subject.keywordAuthor | Magnetic materials | - |
dc.subject.keywordAuthor | Polymeric materials | - |
dc.subject.keywordAuthor | Surface-enhanced Raman scattering | - |
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