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Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier: In vitro transfection

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Authors
Park, In-Kyu; Jiang, Hur-Lin; Cook, Seung-Eun; Cho, Myung-Haing; Kim, Su-Il; Jeong, Hwan-Jeong; Akaike, Toshihiro; Cho, Chong-Su
Issue Date
2004-12
Citation
Archives of Pharmacal Research, Vol.27 No.12, pp.1284-1289
Keywords
galactosylated chitosanpoly(vinyl pyrrolidone)hepatocytegene delivery
Abstract
Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of Ca2+ concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.
ISSN
0253-6269
URI
https://hdl.handle.net/10371/172302
DOI
https://doi.org/10.1007/BF02975895
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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