S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Journal Papers (저널논문_수의학과)
Selective stimulation of caveolae-mediated endocytosis by an osmotic polymannitol-based gene transporter
- Park, Tae-Eun; Kang, Bitna; Kim, You-Kyoung; Zhang, Qiankun; Lee, Won-Seok; Islam, Mohammad Ariful; Kang, Sang-Kee; Cho, Myung-Haing; Choi, Yun-Jaie; Cho, Chong-Su
- Issue Date
- Biomaterials, Vol.33 No.29, pp.7272-7281
- Gene delivery; Caveolae-mediated endocytosis; Polyethylenimine; Polymannitol; Hyperosmotic stress
- Controlling the cellular uptake mechanism and consequent intracellular route of polyplexes is important to improve the transfection efficiency of the non-viral gene delivery. Here, we report a new non-viral vector, polymannitol-based gene transporter (PMT), generated by crosslinking low molecular weight polyethylenimine with mannitol diacrylate, which has low cytotoxicity and good transfection efficiency. Interestingly, the uptake pathway of PMT/DNA complexes was shifted into caveolae-mediated endocytosis, avoiding lysosomal degradation. The mechanism of increased caveolae-mediated endocytosis of PMT/DNA complexes was found to be correlated with mechanosensing signal transduction by the hyperosmotic polymannitol part. Our results suggested that PMT, polymannitol-based gene transporter, is a safe and efficient gene delivery system with a well-modulated uptake pathway and intracellular route for gene therapy. (C) 2012 Elsevier Ltd. All rights reserved.
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