Browse

Accelerated gene transfer through a polysorbitol-based transporter mechanism

Cited 40 time in Web of Science Cited 39 time in Scopus
Authors
Islam, Mohammad Ariful; Yun, Cheol-Heui; Choi, Yun-Jaie; Shin, Ji-Young; Arote, Rohidas; Jiang, Hu-Lin; Kang, Sang-Kee; Nah, Jae-Woon; Park, In-Kyu; Cho, Myung-Haing; Cho, Chong-Su
Issue Date
2011-12
Citation
Biomaterials, Vol.32 No.36, pp.9908-9924
Keywords
Gene transferPolysorbitolTransporterCOX-2 inhibitorAccelerated transfection
Abstract
Here we report an accelerated gene transfer through a polysorbitol-based osmotically active transporter (PSOAT) that shows several surprising results through interesting mechanisms. The nano-sized and well-complexed PSOAT/DNA particles are less toxic, stable at serum and show no aggregation after lyophilization due to their polysorbitol backbone. The transfection is remarkably accelerated both in vitro and in vivo, presumably due to a transporter mechanism of PSOAT in spite of possibility of reduction of transfection by many hydroxyl groups in the transporter. PSOAT possesses a transporter mechanism owing to its polysorbitol backbone, which enhances cellular uptake by exerting polysorbitol transporter activity, thus accelerates gene transfer to cells because transfection ability of PSOAT is drastically reduced in the presence of a cyclooxygenase (COX)-2-specific inhibitor, which we have reported as an inhibitor of the transporter to cells. Moreover, the gene expression is found to be enhanced by hyperosmotic activity and buffering capacity due to polysorbitol and polyethylenimine backbone of PSOAT, respectively. The polysorbitol in PSOAT having polyvalency showed more efficiency in accelerating gene transfer capability than monovalent sorbitol. The above interesting mechanisms display PSOAT as a remarkably potential system to deliver therapeutic (small interfering RNA) and diagnostic agents for effective treatment of cancer. (C) 2011 Elsevier Ltd. All rights reserved.
ISSN
0142-9612
URI
https://hdl.handle.net/10371/172339
DOI
https://doi.org/10.1016/j.biomaterials.2011.09.013
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse