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Physico-chemical characterization-based safety evaluation of nanocalcium

Cited 14 time in Web of Science Cited 16 time in Scopus
Authors
Jeong, Min Sook; Cho, Hyun Sun; Park, Soo Jin; Song, Kyung Seuk; Ahn, Kyu Sup; Cho, Myung-Haing; Kim, Jun Sung
Issue Date
2013-12
Citation
Food and Chemical Toxicology, Vol.62, pp.308-317
Keywords
NanocalciumGenotoxicityPhysico-chemical characteristicsSpecific surface area
Abstract
Nano- and microcalcium provided from the KFDA were compared in terms of physico-chemical properties. Calcium samples were tested using EF-TEM and X-ray diffractometry to check for size/morphology and crystal formation, respectively. Two samples of nano- and microcalcium were selected for further evaluation by FE-SEM, DLS (nano-size, 200-500 nm; agglomerate, >5 mu m; micro-size, 1.5-30 mu m), and electron spin resonance. Both samples were heterogeneous in size, existed as single crystal and aggregated form, and did not generate reactive oxygen species. The specific surface area of nano- and microcalcium measured by N2 Brunauere Emmette Teller method was 12.90 +/- 0.27 m(2)/g and 1.12 +/- 0.19 m(2)/g, respectively. Inductively coupled plasma optical emission spectrometry analysis revealed the release of 2-3 times more calcium ion from nano- compared to microcalcium at pH 5 and 7. Genotoxicity and acute single-dose and repeated-dose 14-day oral toxicity testing in SD rats performed to evaluate the safety of nanocalcium did not reveal toxicity. However, long-term monitoring will be required for an unequivocal conclusion. A nanocalcium dose of 1 g/kg is recommended as the maximum dose for repeated dose 13-week oral toxicity. Further studies could provide details of toxicity of nanocalcium on the repeated dose 13-week oral toxicity test. (C) 2013 Elsevier Ltd. All rights reserved.
ISSN
0278-6915
URI
https://hdl.handle.net/10371/172383
DOI
https://doi.org/10.1016/j.fct.2013.08.024
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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