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Chitosan-graft-polyethylenimine for Akt1 siRNA delivery to lung cancer cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jere, Dhananjay | - |
dc.contributor.author | Jiang, Hu-Lin | - |
dc.contributor.author | Kim, You-Kyoung | - |
dc.contributor.author | Arote, Rohidas | - |
dc.contributor.author | Choi, Yun-Jaie | - |
dc.contributor.author | Yun, Cheol-Heui | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.contributor.author | Cho, Chong-Su | - |
dc.date.accessioned | 2021-01-31T08:43:57Z | - |
dc.date.available | 2021-01-31T08:43:57Z | - |
dc.date.created | 2018-01-22 | - |
dc.date.issued | 2009-08 | - |
dc.identifier.citation | International Journal of Pharmaceutics, Vol.378 No.1-2, pp.194-200 | - |
dc.identifier.issn | 0378-5173 | - |
dc.identifier.other | 25280 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172403 | - |
dc.description.abstract | Efficient delivery of small interfering RNA (siRNA) remains a challenging task in RNA interference (RNAi) studies. In this study, we used chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer composed of chitosan and low molecular weight polyethylenimine (PEI) for the delivery of siRNA. The CHI-g-PEI carrier formed stable complexes with siRNA with compact spherical morphology. CHI-g-PEI delivered EGFP siRNA (siGFP) silenced EGFP expression nearly 2.5 folds higher than PEI25K at 50 pM siGFP concentration. Cell viability was found to be 2 folds high with CHI-g-PEI carrier than PEI25K. Also, our CHI-g-PEI carrier efficiently delivered Akt1 siRNA (siAkt) and thereby silenced onco-protein Akt1. Silencing of this crucial cell survival protein significantly reduced the lung cancer cell survival and proliferation. Additionally, Akt1 protein knock-down decreased A549 cell malignancy and metastasis. These findings suggest that the CHI-g-PEI carrier efficiently and safely delivered siRNA. Moreover, CHI-g-PEI mediated Akt1 siRNA delivery may immerge as a viable approach for lung cancer treatment. (C) 2009 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Chitosan-graft-polyethylenimine for Akt1 siRNA delivery to lung cancer cells | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.1016/j.ijpharm.2009.05.046 | - |
dc.citation.journaltitle | International Journal of Pharmaceutics | - |
dc.identifier.wosid | 000269163600029 | - |
dc.identifier.scopusid | 2-s2.0-67650608146 | - |
dc.citation.endpage | 200 | - |
dc.citation.number | 1-2 | - |
dc.citation.startpage | 194 | - |
dc.citation.volume | 378 | - |
dc.identifier.sci | 000269163600029 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Arote, Rohidas | - |
dc.contributor.affiliatedAuthor | Choi, Yun-Jaie | - |
dc.contributor.affiliatedAuthor | Yun, Cheol-Heui | - |
dc.contributor.affiliatedAuthor | Cho, Myung-Haing | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CELLULAR-SURVIVAL | - |
dc.subject.keywordPlus | GENE CARRIER | - |
dc.subject.keywordPlus | ADENOCARCINOMA | - |
dc.subject.keywordPlus | VIVO | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | Polymeric carrier | - |
dc.subject.keywordAuthor | Non-viral vector | - |
dc.subject.keywordAuthor | SiRNA delivery | - |
dc.subject.keywordAuthor | Akt | - |
dc.subject.keywordAuthor | Chitosan | - |
dc.subject.keywordAuthor | Polyethylenimine | - |
dc.subject.keywordAuthor | Lung cancer | - |
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