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Gene Expression and Pulmonary Toxicity of Chitosan-graft-Polyethylenimine as Aerosol Gene Carrier

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Authors
Kwon, Jung-Taek; Jiang, Hu-Lin; Minai-Tehrani, Arash; Woo, Chang Gyu; Choi, Mansoo; Cho, Chong-Su; Kim, Yeon-Soo; Cho, Myung-Haing
Issue Date
2013-03
Citation
Iranian Journal of Pharmaceutical Research, Vol.12 No.2, pp.281-286
Keywords
Aerosol deliveryChitosanGene carrierPolyethyleniminePulmonary toxicity
Abstract
Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for the improvement of low transfection efficiency of chitosan. The present study aims to test the pulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposed to aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHI-g-PEI complexes for 30 min during the development of our nose-only exposure chamber (NOEC) system. CHI-g-PEI-mediated aerosol delivery demonstrated 15.65% enhancement of the fluorescence intensity. Compared to PEI, CHI-g-PEI showed no significant pulmonary toxicity. In summary, using CHI-g-PEI is safe and shows high transfection in aerosol gene delivery to animals, and enhanced efficiency was achieved through our aerosol gene delivery system. Therefore, CHI-g-PEI and this system would be applicable to future study for aerosol gene therapy.
ISSN
1735-0328
URI
https://hdl.handle.net/10371/172407
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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