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Highty efficient gene transfer with degradabte poty(ester arnine) based on poty(ethytene gtycol) diacrytate and potyethytenimine in vitro and in vivo

Cited 41 time in Web of Science Cited 41 time in Scopus
Authors
Park, Mi Ran; Kim, Hyon Woo; Hwang, Chang Sun; Han, Ki Ok; Choi, Yun Jaie; Song, Soo Chang; Cho, Myung-Haing; Cho, Chong Su
Issue Date
2008-02
Citation
JOURNAL OF GENE MEDICINE, Vol.10 No.2, pp.198-207
Keywords
polyethyleniminepoly(ethylene glycol)poly(ester amine)nonviral gene delivery
Abstract
Background Polyethylenimine (PEI) is toxic although it is one of the most successful and widely used gene delivery polymers with the aid of the proton sponge effect. Therefore, development of new novel gene delivery carriers having high efficiency with less toxicity is necessary. Methods In this study, a degradable poly(ester amine) carrier based on poly(ethylene glycol) diacrylate (PEGDA) and low molecular weight linear PEI was prepared. Furthermore, we compared the gene expression of the polymer/DNA complexes using two delivery methods: intravenous administration as an invasive method and aerosol as a non-invasive method. Results The synthesized polymer had a relatively small molecular weight (MW = 7980) with 25 h half-life in vitro. The polymer/DNA complexes were formed at an N/P ratio of 9. The particle sizes and zeta-potentials of the complexes were dependent on N/P ratio. Compared to PEI 25K, the newly synthesized polymer exhibited high transfection efficiency with low toxicity. Poly(ester amine)-mediated gene expression in the lung and liver was higher than that of the conventional PEI carrier. Interestingly, non-invasive aerosol delivery induced higher gene expression in all organs compared to intravenous method in an in vivo mice study. Such an expressed gene via a single aerosol administration in the lung and liver remained unchanged for 7 days. Conclusions Our study demonstrates that poly(ester amine) may be applied as an useful gene carrier. Copyright (C) 2007 John Wiley & Sons, Ltd.
ISSN
1099-498X
URI
https://hdl.handle.net/10371/172429
DOI
https://doi.org/10.1002/jgm.1139
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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