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Serum immunoglobulin fused interferon-alpha inhibited tumor growth in athymic mice bearing colon 26 adenocarcinoma cells

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Authors
Kim, Jun Sung; Yu, Kyeing Nam; Noh, Mi Suk; Woo, Min-Ah; Park, Sung-Jin; Park, Jin Hong; Hua, Jin; Cho, Hyun Sun; Hwang, Soon Kyung; Lee, Eun Sun; Chung, Youn-Sun; Choi, In-Young; Kwon, Se-Chang; Cho, Myung-Haing
Issue Date
2008-03
Citation
Journal of Veterinary Science, Vol.9 No.1, pp.45-50
Keywords
adenocarcinoma cellinterferonserum immunoglobulintumor growth inhibition
Abstract
Interferon (IFN) has therapeutic potential for a wide range of infectious and proliferative disorders. However, the half-life of IFN is too short to have a stable therapeutic effect. To overcome this problem, serum immunoglobulin has been fused to IFN. In this study, the efficacy of serum immunoglobulin fused INFs (si-IFN1 and si-IFN2) was evaluated on athymic mice bearing colon 26 adenocarcinoma cells. Seven days after the implantation of tumor cells, each group of mice was injected once a week with si-IFN1 and si-IFN2 at two different concentrations (10 x : 30 mu g/kg and 50 x : 150 mu g/kg). A slight anti-tumoral effect was observed in all 10 x groups compared to the control. In the 50 x groups, however, si-IFN1 and si-IFN2 showed significant anti- tumoral effects compared to the control. To gain more information on the mechanisms associated with the decrease of tumor size, a Western blot assay of apoptosis-related molecules was performed. The protein expression of cytochrome c, caspase 9, 6, and 3 were increased by si-IFN1 and si-IFN2. These 2 IFNs also increased the expressions of p53, p21, Bax and Bad. Interestingly, si-IFN1 and si-IFN2 decreased the expression of VEGF-beta. Taken together, serum immunoglobulin fused IFNs increased therapeutic efficacy under current experimental condition.
ISSN
1229-845X
URI
https://hdl.handle.net/10371/172459
DOI
https://doi.org/10.4142/jvs.2008.9.1.45.
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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