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Lentiviral vector-mediated shRNA against AIMP2-DX2 suppresses lung cancer cell growth through blocking glucose uptake

Cited 9 time in Web of Science Cited 8 time in Scopus
Authors
Chang, Seung-Hee; Chung, Youn-Sun; Hwang, Soon-Kyung; Kwon, Jung-Taek; Minai-Tehrani, Arash; Kim, Sunghoon; Park, Seung Bum; Kim, Yeon-Soo; Cho, Myung-Haing
Issue Date
2012-06
Citation
Molecules and Cells, Vol.33 No.6, pp.553-562
Keywords
AIMP2-DX2EGFR/MAPK signaling pathwayglucose uptakegluts
Abstract
Aminoacyl-tRNA synthetases [ARS]-interacting multifunctional protein 2 (AIMP2) has been implicated in the control of cell fate and lung cell differentiation. A variant of AIMP2 lacking exon 2 (AIMP2-DX2) is expressed in different cancer cells. We previously studied the expression level of AIMP2-DX2 in several lung cell lines and reported elevated expression levels of AIMP2-DX2 in NCI-H460 and NCI-H520. Here, we report that the suppression of AIMP2-DX2 by lentivirus mediated short hairpin (sh)RNA (sh-DX2) decreased the rate of glucose uptake and glucose transporters (Gluts) in NCI-H460 cells. Down-regulation of AIMP2-DX2 reduced glycosyltransferase (GnT)-V in the Golgi apparatus, while inducing the GnT-V antagonist GnT-III. Down-regulation of AIMP2-DX2 also suppressed the epidermal growth factor receptor/mitogen activated protein kinase (EGFR/MAPK) signaling pathway, leading to the decrease of the proliferation marker Ki-67 expression in nuclei. Furthermore, dual luciferase activity reduced capdependent protein translation in cells infected with sh-DX2. These results suggest that AIMP2-DX2 may be a relevant therapeutic target for lung cancer, and that the sh-DX2 lentiviral system can be an appropriate method for lung cancer therapy.
ISSN
1016-8478
URI
https://hdl.handle.net/10371/172476
DOI
https://doi.org/10.1007/s10059-012-2269-2
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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