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Hybrid of baculovirus and galactosylated PEI for efficient gene carrier

Cited 17 time in Web of Science Cited 21 time in Scopus
Authors
Kim, You-Kyoung; Choi, Jae Young; Jiang, Hu-Lin; Arote, Rohidas; Jere, Dhananjay; Cho, Myung-Haing; Je, Yeon Ho; Cho, Chong-Su
Issue Date
2009-04
Citation
Virology, Vol.387 No.1, pp.89-97
Keywords
Gene therapyTargeted deliveryHybrid systemBaculovirusGalactosylated PEI
Abstract
Baculovirus, containing an appropriate eukaryotic promoter, is considered an attractive approach for an efficient and safe gene delivery vehicle. However, the drawbacks of baculovirus, Such as the lack of specificity and the inactivation of baculovirus by the complement system in human serum, negatively affect efficient gene delivery. Therefore, a hybrid system utilizing the positive aspects of both vital and non-viral vector systems would be useful to overcome the obstacles of either system alone. In this study, we constructed a hybrid system composed of baculovirus (B) and galactosylated polyethylenimine (GP)/DNA complexes through electrostatic interaction. The resulting GP/B hybrid had suitable physicochemical properties and low cytotoxicity for use in gene therapy. Furthermore, the GP/B significantly enhanced transduction efficiency and showed good cell-specificity compared to either viral or non-viral vector systems. These results suggest that the GP/B hybrid system can be used in gene therapy to enhance transduction efficiency and hepatocyte specificity. (C) 2009 Elsevier Inc. All rights reserved.
ISSN
0042-6822
URI
https://hdl.handle.net/10371/172533
DOI
https://doi.org/10.1016/j.virol.2009.02.001
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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