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Esculetin Suppresses Proliferation of Human Colon Cancer Cells by Directly Targeting beta-Catenin

Cited 39 time in Web of Science Cited 44 time in Scopus
Authors
Lee, Sung-Young; Lim, Tae-Gyu; Chen, Hanyong; Jung, Sung Keun; Lee, Hyo-Jeong; Lee, Mee-Hyun; Kim, Dong Joon; Shin, Aram; Lee, Ki Won; Bode, Ann M.; Surh, Young-Joon; Dong, Zigang
Issue Date
2013-12
Citation
Cancer Prevention Research, Vol.6 No.12, pp.1356-1364
Abstract
The Wnt pathway is a promising therapeutic and preventive target in various human cancers. The transcriptional complex of beta-catenin-T-cell factor (Tcf), a key mediator of canonical Wnt signaling, has been implicated in human colon cancer development. Current treatment of colon cancer depends on traditional cytotoxic agents with limited effects. Therefore, the identification of natural compounds that can disrupt the beta-catenin-TcF complex to suppress cancer cell growth with fewer adverse side effects is needed. To identify compounds that inhibit the association between beta-catenin and Tcf, we used computer docking to screen a natural compound library. Esculetin, also known as 6,7-dihydroxycoumarin, is a derivative of coumarin and was identified as a potential small-molecule inhibitor of the Wnt-beta-catenin pathway. We then evaluated the effect of esculetin on the growth of various human colon cancer cell lines and its effect on Wnt-beta-catenin signaling in cells and in an embryonic model. Esculetin disrupted the formation of the beta-catenin-Tcf complex through direct binding with the Lys312, Gly307, Lys345, and Asn387 residues of beta-catenin in colon cancer cells. In addition, esculetin effectively decreased viability and inhibited anchorage-independent growth of colon cancer cells. Esculetin potently antagonized the cellular effects of beta-catenin-dependent activity, and in vivo treatment with esculetin suppressed tumor growth in a colon cancer xenograft mouse model. Our data indicate that the interaction between esculetin and beta-catenin inhibits the formation of the beta-catenin-Tcf complex, which could contribute to esculetin's positive therapeutic and preventive effects against colon carcinogenesis. (C) 2013 AACR.
ISSN
1940-6207
URI
https://hdl.handle.net/10371/172657
DOI
https://doi.org/10.1158/1940-6207.CAPR-13-0241
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Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Molecular and Biopharmaceutical Sciences (분자의학 및 바이오제약학과)Journal Papers (저널논문_분자의학 및 바이오제약학과)
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