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MAPK signaling is involved in camptothecin-induced cell death

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Authors
Lee, Seongeun; Lee, Ho-Soon; Baek, Myunginq; Lee, Dae-Yeon; Bang, Yung-Jue; Cho, Hae-Nyun; Lee, Yun-Sil; Ha, Ji-Hong; Kim, Hae-Yeong; Jeong, Doo-Il
Issue Date
2002-12
Citation
Molecules and Cells, Vol.14 No.3, pp.348-354
Keywords
AGSapoptosiscamptothecinMAPK
Abstract
Camptothecin, a topoisomerase I inhibitor, is a well-known anticancer drug. However, its mechanism has not been well studied in human gastric cancer cell lines. Camptothecin induced apoptotic cell death in human gastric cancer cell line AGS. Z-VAD-fmk, pan-caspase inhibitor, blocked apoptotic phenotypes induced by camptothecin suggesting that caspases are involved in camptothecin-induced cell death. An inhibitor of caspase-6 or -8 or -9 did not prevent cell death by camptothecin. Various protease inhibitors failed to prevent camptothecin-induced cell death. These results suggest that only few caspases are involved in camptothecin-induced cell death. Camptothecin induced phosphorylation of ERK1/2, JNK, and p38 MAPK, in a dose and time-dependent manner in AGS. Z-VAD-fmk did not affect MAPK signaling induced by camptothecin suggesting that caspase signaling occurs downstream of MAPK signaling. Blocking of p38 MAPK, but not ERK1/2, resulted in partial inhibition of cell death and PARP cleavage by camptothecin in AGS. Taken together, MAPK signaling is associated with apoptotic cell death by camptothecin.
ISSN
1016-8478
URI
https://hdl.handle.net/10371/172947
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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