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Mutation of ras oncogene in gastric adenocarcinoma: Association with histological phenotype

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Authors
Kim, TY; Bang, Yung-Jue; Kim, WS; Kang, SH; Lee, KU; Choe, KJ; Kim, NK
Issue Date
1997-03
Citation
Anticancer Research, Vol.17 No.2B, pp.1335-1339
Keywords
gastric carcinomaras oncogenepoint mutationpolymerase chain reactionsequencingdifferentiation
Abstract
In order to explore the role of ras oncogene in gastric carcinomas from Korean patients, we examined the frequency of point mutations all three ras oncogenes (Ki-, Ha-, and N-ras). A total of 57 DNA samples were prepared from 3 gastric carcinoma cell lines, 10 malignant ascites, and 44 frozen gastric tremor tissues. Exons I and 2 of each ras oncogene were amplified by polymerase chain reaction (PCR), and analyzed by single strand conformation polymorphism (SSCP) and direct sequencing. Mutated ms genes were detected in 6 out of 57 samples (10%). One cell line and 2 tumors showed a mutation at exon 1 of Ki-ras. N-ras mutations were also detected at exon I of 3 tumors. Histologically, all the ras mutation cases exhibited a diffuse phenotype. In summary, we performed a comprehensive analysis to investigate the mutation of all three ms oncogenes in gastric carcinoma. The results demonstrate infrequent mutations of Ki-and N-ras which may favor the development of diffuse type gastric carcinomas, implicating a different genetic pathway in diffuse and intestinal type gastric carcinomas.
ISSN
0250-7005
URI
https://hdl.handle.net/10371/172965
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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