S-Space College of Medicine/School of Medicine (의과대학/대학원) Internal Medicine (내과학전공) Journal Papers (저널논문_내과학전공)
Mutation of ras oncogene in gastric adenocarcinoma: Association with histological phenotype
- Kim, TY; Bang, Yung-Jue; Kim, WS; Kang, SH; Lee, KU; Choe, KJ; Kim, NK
- Issue Date
- Anticancer Research, Vol.17 No.2B, pp.1335-1339
- gastric carcinoma; ras oncogene; point mutation; polymerase chain reaction; sequencing; differentiation
- In order to explore the role of ras oncogene in gastric carcinomas from Korean patients, we examined the frequency of point mutations all three ras oncogenes (Ki-, Ha-, and N-ras). A total of 57 DNA samples were prepared from 3 gastric carcinoma cell lines, 10 malignant ascites, and 44 frozen gastric tremor tissues. Exons I and 2 of each ras oncogene were amplified by polymerase chain reaction (PCR), and analyzed by single strand conformation polymorphism (SSCP) and direct sequencing. Mutated ms genes were detected in 6 out of 57 samples (10%). One cell line and 2 tumors showed a mutation at exon 1 of Ki-ras. N-ras mutations were also detected at exon I of 3 tumors. Histologically, all the ras mutation cases exhibited a diffuse phenotype. In summary, we performed a comprehensive analysis to investigate the mutation of all three ms oncogenes in gastric carcinoma. The results demonstrate infrequent mutations of Ki-and N-ras which may favor the development of diffuse type gastric carcinomas, implicating a different genetic pathway in diffuse and intestinal type gastric carcinomas.
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