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Mutation of ras oncogene in gastric adenocarcinoma: Association with histological phenotype
Cited 15 time in
Web of Science
Cited 15 time in Scopus
- Authors
- Issue Date
- 1997-03
- Citation
- Anticancer Research, Vol.17 No.2B, pp.1335-1339
- Abstract
- In order to explore the role of ras oncogene in gastric carcinomas from Korean patients, we examined the frequency of point mutations all three ras oncogenes (Ki-, Ha-, and N-ras). A total of 57 DNA samples were prepared from 3 gastric carcinoma cell lines, 10 malignant ascites, and 44 frozen gastric tremor tissues. Exons I and 2 of each ras oncogene were amplified by polymerase chain reaction (PCR), and analyzed by single strand conformation polymorphism (SSCP) and direct sequencing. Mutated ms genes were detected in 6 out of 57 samples (10%). One cell line and 2 tumors showed a mutation at exon 1 of Ki-ras. N-ras mutations were also detected at exon I of 3 tumors. Histologically, all the ras mutation cases exhibited a diffuse phenotype. In summary, we performed a comprehensive analysis to investigate the mutation of all three ms oncogenes in gastric carcinoma. The results demonstrate infrequent mutations of Ki-and N-ras which may favor the development of diffuse type gastric carcinomas, implicating a different genetic pathway in diffuse and intestinal type gastric carcinomas.
- ISSN
- 0250-7005
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