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Establishment and characterization of human gastric carcinoma cell lines
DC Field | Value | Language |
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dc.contributor.author | Park, Jae‐Gahb | - |
dc.contributor.author | Yang, Han‐Kwang | - |
dc.contributor.author | Kim, Woo Ho | - |
dc.contributor.author | Chung, June‐Key | - |
dc.contributor.author | Kang, Myung‐Soo | - |
dc.contributor.author | Lee, Jae‐Ho | - |
dc.contributor.author | Oh, Jae Hwan | - |
dc.contributor.author | Park, Hyun‐Sook | - |
dc.contributor.author | Yeo, Kyong‐Sook | - |
dc.contributor.author | Kang, Shin Hyuck | - |
dc.contributor.author | Song, Sang‐Yong | - |
dc.contributor.author | Kang, Yun Kyung | - |
dc.contributor.author | Bang, Yung-Jue | - |
dc.contributor.author | Kim, Yong Il | - |
dc.contributor.author | Kim, Jin‐Pok | - |
dc.date.accessioned | 2021-01-31T11:12:20Z | - |
dc.date.available | 2021-01-31T11:12:20Z | - |
dc.date.created | 2020-12-23 | - |
dc.date.issued | 1997-02 | - |
dc.identifier.citation | International Journal of Cancer, Vol.70 No.4, pp.443-449 | - |
dc.identifier.issn | 0020-7136 | - |
dc.identifier.other | 119610 | - |
dc.identifier.uri | https://hdl.handle.net/10371/173106 | - |
dc.description.abstract | We report 8 newly established gastric-carcinoma cell lines (SNU-216, 484, 520, 601, 620, 638, 668, 719) from Korean patients, Morphologic study was carried out using light and electron microscopes. CEA, alpha FP, and CA 19-9 and TPA in supernatant and in cell lysate were measured by radioimmunoassay. p53 and c-Ki-ras gene mutations were screened and confirmed by sequencing. The cell lines, derived from tumors with moderate differentiation, grew as a diffuse monolayer, and those from tumors with poor differentiation and minimal desmoplasia grew exclusively as non-adherent. Out of the 8 gastric-cancer cell lines, 5 had detectable levels of CEA both in supernatant and in cell lysate; there was no expression or secretion of alpha FP in these cells; 4 cell lines showed high levels of CA 19-9 in cell pellets, All cell lines except SNU-484 had high concentrations of TPA both in cell lysate and in supernatants. p53 mutation was found in 6 cell lines (75%): 2 (SNU-216 and SNU-668) had mutations in exon 6, and other 3 in exon 8. The c-Ki-ras mutation was found in 2 cell lines (25%), SNU-601 and SNU-668. The former showed GGT-to-GAT transition mutation at codon 12, while the latter showed CAA-to-AAA transversion mutation at codon 61. DNA profiles using restriction endonuclease Hinfl and polymorphic DNA probes ChdTC-15 and ChdTC-[14 showed different unique patterns; which suggests that these cell lines are unique and not cross-contaminated. We believe that the newly characterized gastric-cancer cell lines presented in this paper will provide a useful in vitro model for studies related to human gastric cancer. (C) 1997 Wiley-Liss, Inc. | - |
dc.language | 영어 | - |
dc.publisher | John Wiley & Sons Inc. | - |
dc.title | Establishment and characterization of human gastric carcinoma cell lines | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 방영주 | - |
dc.identifier.doi | 10.1002/(SICI)1097-0215(19970207)70:4<443::AID-IJC12>3.0.CO;2-G | - |
dc.citation.journaltitle | International Journal of Cancer | - |
dc.identifier.wosid | A1997WF99000012 | - |
dc.identifier.scopusid | 2-s2.0-0031038107 | - |
dc.citation.endpage | 449 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 443 | - |
dc.citation.volume | 70 | - |
dc.identifier.sci | A1997WF99000012 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Jae‐Gahb | - |
dc.contributor.affiliatedAuthor | Yang, Han‐Kwang | - |
dc.contributor.affiliatedAuthor | Kim, Woo Ho | - |
dc.contributor.affiliatedAuthor | Chung, June‐Key | - |
dc.contributor.affiliatedAuthor | Bang, Yung-Jue | - |
dc.contributor.affiliatedAuthor | Kim, Yong Il | - |
dc.contributor.affiliatedAuthor | Kim, Jin‐Pok | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | N-RAS GENE | - |
dc.subject.keywordPlus | P53 GENE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | COMMON | - |
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