Browse

Toll-like receptor 2 downregulation and cytokine dysregulation predict mortality in patients with Staphylococcus aureus bacteremia

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Kim, Nak-Hyun; Sung, Ji Yeon; Choi, Yoon Jung; Choi, Su-Jin; Ahn, Soyeon; Ji, Eunjeong; Kim, Moonsuk; Kim, Chung Jong; Song, Kyoung-Ho; Choe, Pyoeng Gyun; Park, Wan Beom; Kim, Eu Suk; Park, Kyoung Un; Kim, Nam-Joong; Oh, Myoung-don; Kim, Hong Bin
Issue Date
2020-11-30
Publisher
BMC
Citation
BMC Infectious Diseases. 2020 Nov 30;20(1):901
Keywords
Staphylococcus aureusBacteremiaToll-like receptor 2 (TLR2)Cytokines
Abstract
Background
Staphylococcus aureus bacteremia (SAB) presents heterogeneously, owing to the differences in underlying host conditions and immune responses. Although Toll-like receptor 2 (TLR2) is important in recognizing S. aureus, its function during S. aureus infection remains controversial. We aimed to examine the association of TLR2 expression and associated cytokine responses with clinical SAB outcomes.

Methods
Patients from a prospective SAB cohort at two tertiary-care medical centers were enrolled. Blood was sampled at several timepoints (≤5 d, 6–9 d, 10–13 d, 14–19 d, and ≥ 20 d) after SAB onset. TLR2 mRNA levels were determined via real-time PCR and serum tumor necrosis factor [TNF]-α, interleukin [IL]-6, and IL-10 levels were analyzed with multiplex-high-sensitivity electrochemiluminescent ELISA.

Results
TLR2 levels varied among 59 SAB patients. On days 2–5, TLR2 levels were significantly higher in SAB survivors than in healthy controls (p = 0.040) and slightly but not significantly higher than non-survivors (p = 0.120), and SAB patients dying within 7 d had lower TLR2 levels than survivors (P = 0.077) although statistically insignificant. IL-6 and IL-10 levels were significantly higher in non-survivors than in survivors on days 2–5 post-bacteremia (P = 0.010 and P = 0.021, respectively), and those dying within 7 d of SAB (n = 3) displayed significantly higher IL-10/TNF-α ratios than the survivors did (P = 0.007).

Conclusion
TLR2 downregulation and IL-6 and IL-10 concentrations suggestive of immune dysregulation during early bacteremia may be associated with mortality from SAB. TLR2 expression levels and associated cytokine reactions during early-phase SAB may be potential prognostic factors in SAB, although larger studies are warranted.
ISSN
1471-2334
Language
English
URI
http://hdl.handle.net/10371/173354
DOI
https://doi.org/10.1186/s12879-020-05641-z
Files in This Item:
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse