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The mutation of BCOR is highly recurrent and oncogenic in mature T-cell lymphoma

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dc.contributor.authorKang, Jin Hyun-
dc.contributor.authorLee, Seung Ho-
dc.contributor.authorLee, Jawon-
dc.contributor.authorChoi, Murim-
dc.contributor.authorCho, Junhun-
dc.contributor.authorKim, Seok Jin-
dc.contributor.authorKim, Won Seog-
dc.contributor.authorKo, Young Hyeh-
dc.contributor.authorYoo, Hae Yong-
dc.date.accessioned2021-02-23T06:37:31Z-
dc.date.available2021-02-23T15:39:13Z-
dc.date.issued2021-01-19-
dc.identifier.citationBMC Cancer. 2021 Jan 19;21(1):82ko_KR
dc.identifier.issn1471-2407-
dc.identifier.urihttps://hdl.handle.net/10371/173378-
dc.description.abstractBackground
BCOR acts as a corepressor of BCL6, a potent oncogenic protein in cancers of the lymphoid lineage. We have found the recurrent somatic mutation of BCOR occurred in mature T-cell lymphoma (TCL). The role of BCOR mutation in lymphoid malignancies is unknown.

Methods
Lymphoma patient samples were analyzed to identify missense mutations in BCOR using Sanger sequencing. Transfection, RNA interference, immunoprecipitation, western blotting, cell proliferation, cytokine assays and quantitative real-time PCR were employed to determine the functional relevance of the novel K607E mutation in BCOR. The significant transcriptional changes were analyzed by performing DNA microarray profiling in cells expressing BCOR K607E mutant.

Results
One hundred thirty-seven lymphoma patient samples were analyzed to identify K607E mutation of the BCOR gene. The BCOR K607E mutation was identified in 15 of 47 NK/T cell lymphoma cases (31.9%), 2 of 18 angioimmunoblastic T-cell lymphoma cases (11.1%), 10 of 30 peripheral T-cell lymphoma, not otherwise specified cases (33.3%), and 13 of 42 diffuse large B-cell lymphoma cases (30.9%). Molecular analysis of BCOR K607E mutation revealed that compared to the wild-type BCOR, the mutant BCOR bound to the BCL6, PCGF1, and RING1B proteins with lesser affinity. Ectopic expression of BCOR K607E mutant significantly enhanced cell proliferation, AKT phosphorylation and the expression of interleukin-2 (IL-2) with up-regulated expression of HOX and S100 protein genes in T cells. BCOR silencing also significantly enhanced cell proliferation, AKT phosphorylation, and IL-2 production.

Conclusions
Functional analyses indicated that K607E mutation of BCOR is oncogenic in nature and can serve as a genetic marker of T-cell lymphoma.
ko_KR
dc.description.sponsorshipThis work was supported by grants from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI14C3414 to YHK and HYY, HI14C2331 to HYY). This study was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF2018R1D1A1B07043696 to JHK). There was no role of the funding body in the design of the study and collectioko_KR
dc.language.isoenko_KR
dc.publisherBMCko_KR
dc.subjectBCOR-
dc.subjectT-cell lymphoma-
dc.subjectBCL6-
dc.subjectGene mutation-
dc.subjectHOX-
dc.subjectS100 protein-
dc.titleThe mutation of BCOR is highly recurrent and oncogenic in mature T-cell lymphomako_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor강진현-
dc.contributor.AlternativeAuthor이승호-
dc.contributor.AlternativeAuthor이재원-
dc.contributor.AlternativeAuthor최무림-
dc.contributor.AlternativeAuthor조준훈-
dc.contributor.AlternativeAuthor김석진-
dc.contributor.AlternativeAuthor김원석-
dc.contributor.AlternativeAuthor고영혜-
dc.contributor.AlternativeAuthor유해용-
dc.identifier.doi10.1186/s12885-021-07806-8-
dc.citation.journaltitleBMC Cancerko_KR
dc.language.rfc3066en-
dc.rights.holderThe Author(s)-
dc.date.updated2021-01-27T09:55:46Z-
dc.citation.number1ko_KR
dc.citation.startpage82ko_KR
dc.citation.volume21ko_KR
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biomedical Sciences (대학원 의과학과)Journal Papers (저널논문_의과학과)
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