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Production of biologically active human interleukin-10 by Bifidobacterium bifidum BGN4
DC Field | Value | Language |
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dc.contributor.author | Hong, Nayoun | - |
dc.contributor.author | Ku, Seockmo | - |
dc.contributor.author | Yuk, Kyungjin | - |
dc.contributor.author | Johnston, Tony V | - |
dc.contributor.author | Ji, Geun Eog | - |
dc.contributor.author | Park, Myeong Soo | - |
dc.date.accessioned | 2021-03-04T08:15:27Z | - |
dc.date.available | 2021-03-04T17:18:37Z | - |
dc.date.issued | 2021-01-19 | - |
dc.identifier.citation | Microbial Cell Factories. 2021 Jan 19;20(1):16 | ko_KR |
dc.identifier.issn | 1475-2859 | - |
dc.identifier.uri | https://hdl.handle.net/10371/173432 | - |
dc.description.abstract | Background
Bifidobacterium spp. are representative probiotics that play an important role in the health of their hosts. Among various Bifidobacterium spp., B. bifidum BGN4 exhibits relatively high cell adhesion to colonic cells and has been reported to have various in vivo and in vitro bio functionalities (e.g., anti-allergic effect, anti-cancer effect, and modulatory effects on immune cells). Interleukin-10 (IL-10) has emerged as a major suppressor of immune response in macrophages and other antigen presenting cells and plays an essential role in the regulation and resolution of inflammation. In this study, recombinant B. bifidum BGN4 [pBESIL10] was developed to deliver human IL-10 effectively to the intestines. Results The vector pBESIL10 was constructed by cloning the human IL-10 gene under a gap promoter and signal peptide from Bifidobacterium spp. into the E. coli-Bifidobacterium shuttle vector pBES2. The secreted human IL-10 from B. bifidum BGN4 [pBESIL10] was analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western Blotting, and enzyme-linked immunosorbent assay (ELISA). More than 1,473 ± 300ng/mL (n = 4) of human IL-10 was obtained in the cell free culture supernatant of B. bifidum BGN4 [pBESIL10]. This productivity is significantly higher than other previously reported human IL-10 level from food grade bacteria. In vitro functional evaluation of the cell free culture supernatant of B. bifidum BGN4 [pBESIL10] revealed significantly inhibited interleukin-6 (IL-6) production in lipopolysaccharide (LPS)-induced Raw 264.7 cells (n = 6, p < 0.0001) and interleukin-8 (IL-8) production in LPS-induced HT-29 cells (n = 6, p < 0.01) or TNFα-induced HT-29 cells (n = 6, p < 0.001). Conclusion B. bifidum BGN4 [pBESIL10] efficiently produces and secretes significant amounts of biologically active human IL-10. The human IL-10 production level in this study is the highest of all human IL-10 production reported to date. Further research should be pursued to evaluate B. bifidum BGN4 [pBESIL10] producing IL-10 as a treatment for various inflammation-related diseases, including inflammatory bowel disease, rheumatoid arthritis, allergic asthma, and cancer immunotherapy. | ko_KR |
dc.description.sponsorship | This work was carried out with the support of the Ministry of Small and Medium-sized Enterprises(SMEs) and Startups(MSS), Korea, under the Regional Specialized Industry Development Program (R&D, Project number S2848321) supervised by the Korea Institute for Advancement of Technology(KIAT). This work was also supported by a Faculty Research and Creative Activity Committee (FRCAC) Grant (No. 221745) funded by Middle Tennessee State University in the U.S. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Human interleukin-10 | - |
dc.subject | Bifdobacterium bifdum | - |
dc.subject | Secretion | - |
dc.subject | Bioactive | - |
dc.subject | Recombinant | - |
dc.subject | Expression vector | - |
dc.title | Production of biologically active human interleukin-10 by Bifidobacterium bifidum BGN4 | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 홍나윤 | - |
dc.contributor.AlternativeAuthor | 구석모 | - |
dc.contributor.AlternativeAuthor | 육경진 | - |
dc.contributor.AlternativeAuthor | 지근억 | - |
dc.contributor.AlternativeAuthor | 박명수 | - |
dc.identifier.doi | 10.1186/s12934-020-01505-y | - |
dc.citation.journaltitle | Microbial Cell Factories | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2021-01-27T10:02:08Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 16 | ko_KR |
dc.citation.volume | 20 | ko_KR |
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