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Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

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dc.contributor.authorLee, Sohyun-
dc.contributor.authorPark, Nanjoo-
dc.contributor.authorYun, Sujung-
dc.contributor.authorHur, Eunseon-
dc.contributor.authorSong, Jiwon-
dc.contributor.authorLee, Hanna-
dc.contributor.authorKim, Yongsug-
dc.contributor.authorRyu, Sangryeol-
dc.date.accessioned2021-08-11T04:14:36Z-
dc.date.available2021-08-11T13:15:51Z-
dc.date.issued2021-06-01-
dc.identifier.citationGut Pathogens. 2021 Jun 01;13(1):35ko_KR
dc.identifier.issn1757-4749-
dc.identifier.urihttps://hdl.handle.net/10371/174780-
dc.description.abstractNon-typhoidal salmonellosis remains a pressing public health problem worldwide. Quinolones, particularly fluoroquinolones, are widely used to treat various infections, including non-typhoidal salmonellosis, which can be a serious illness. The emergence of fluoroquinolone-resistant Salmonella has resulted in treatment failure and high mortality rates. In this study, we estimated the presence of plasmid-mediated quinolone resistance (PMQR) genes in Salmonella enterica isolated from human salmonellosis patients in South Korea from 2016 to 2019. We evaluated the association of these genes with fluoroquinolone susceptibility. Antimicrobial susceptibility tests for Salmonella isolates were performed using the Vitek II system, and the minimum inhibitory concentrations (MIC) of ciprofloxacin and levofloxacin were determined using the E-test method. Plasmid-mediated quinolone resistance (PMQR) genes were detected by PCR amplification and quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were analyzed following Sanger sequencing of the PCR products. Thirty-four Salmonella strains with reduced susceptibility to fluoroquinolones (ciprofloxacin MIC ≥ 0.125 µg/mL and levofloxacin MIC ≥ 0.25 µg/mL) were selected from 208 human clinical Salmonella isolates. Among them, 22 Salmonella strains harbored one PMQR gene (qnrA, qnrB, or qnrS), and three Salmonella strains carried two PMQR genes (qnrS and aac(6′)-Ib-cr or qnrA and qnrB). qnrS was the most common PMQR gene. Serotyping revealed that Salmonella 4,[5]12:i:- (32.4%, 11/34) and Salmonella Typhimurium (29.4%, 10/34) were the two most predominant serovars, and Multi-locus sequence typing (MLST) showed that ST19 and ST34 were the most frequent sequence types. In conclusion, qnr gene-positive Salmonella 4,[5],12:i:- and Salmonella Typhimurium were the main serovars responsible for reduced susceptibility to fluoroquinolones. Therefore, our findings suggest that PMQR-positive Salmonella strains, which can be isolated from various samples including human, food, and the environment, should be carefully monitored.ko_KR
dc.description.sponsorshipThis research was supported by a Grant (19162MFDS037) from the Ministry of Food and Drug Safety in 2020.ko_KR
dc.language.isoenko_KR
dc.publisherBMCko_KR
dc.subjectSalmonella-
dc.subjectQuinolone resistance-
dc.subjectPlasmid-mediated quinolone resistance-
dc.subjectPMQR-
dc.titlePresence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019ko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor이소현-
dc.contributor.AlternativeAuthor박난주-
dc.contributor.AlternativeAuthor윤수정-
dc.contributor.AlternativeAuthor허은선-
dc.contributor.AlternativeAuthor송지원-
dc.contributor.AlternativeAuthor이하나-
dc.contributor.AlternativeAuthor김용석-
dc.contributor.AlternativeAuthor유상렬-
dc.identifier.doi10.1186/s13099-021-00431-7-
dc.citation.journaltitleGut Pathogensko_KR
dc.language.rfc3066en-
dc.rights.holderThe Author(s)-
dc.date.updated2021-06-06T03:12:29Z-
dc.citation.number1ko_KR
dc.citation.startpage35ko_KR
dc.citation.volume13ko_KR
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