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The SARS-CoV-2 RNA interactome
Cited 71 time in
Web of Science
Cited 75 time in Scopus
- Authors
- Issue Date
- 2021-07
- Publisher
- Cell Press
- Citation
- Molecular Cell, Vol.81 No.13, pp.2838-2850
- Abstract
- SARS-CoV-2 is an RNA virus whose success as a pathogen relies on its abilities to repurpose host RNA -binding proteins (RBPs) and to evade antiviral RBPs. To uncover the SARS-CoV-2 RNA interactome, we here develop a robust ribonucleoprotein (RNP) capture protocol and identify 109 host factors that directly bind to SARS-CoV-2 RNAs. Applying RNP capture on another coronavirus, HCoV-OC43, revealed evolutionarily conserved interactions between coronaviral RNAs and host proteins. Transcriptome analyses and knockdown experiments delineated 17 antiviral RBPs, including ZC3HAV1, TRIM25, PARP12, and SHFL, and 8 pro viral RBPs, such as EIF3D and CSDE1, which are responsible for co-opting multiple steps of the mRNA life cycle. This also led to the identification of LARP1, a downstream target of the mTOR signaling pathway, as an antiviral host factor that interacts with the SARS-CoV-2 RNAs. Overall, this study provides a comprehensive list of RBPs regulating coronaviral replication and opens new avenues for therapeutic interventions.
- ISSN
- 1097-2765
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