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Multi-enzymatic cascade reactions with Escherichia coli-based modules for synthesizing various bioplastic monomers from fatty acid methyl esters
DC Field | Value | Language |
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dc.contributor.author | Yoo, Hee-Wang | - |
dc.contributor.author | Jung, Hyunsang | - |
dc.contributor.author | Sarak, Sharad | - |
dc.contributor.author | Kim, Ye Chan | - |
dc.contributor.author | Park, Beom Gi | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.contributor.author | Patil, Mahesh D. | - |
dc.contributor.author | Yun, Hyungdon | - |
dc.date.accessioned | 2022-05-04T01:41:55Z | - |
dc.date.available | 2022-05-04T01:41:55Z | - |
dc.date.created | 2022-03-14 | - |
dc.date.issued | 2022-03 | - |
dc.identifier.citation | Green Chemistry, Vol.24 No.5, pp.2222-2231 | - |
dc.identifier.issn | 1463-9262 | - |
dc.identifier.uri | https://hdl.handle.net/10371/179295 | - |
dc.description.abstract | Multi-enzymatic cascade reaction systems were designed to generate biopolymer monomers using Escherichia coli-based cell modules, capable of carrying out one-pot reactions. Three cell-based modules, including a omega-hydroxylation module (Cell-H-m) to convert fatty acid methyl esters (FAMEs) to omega-hydroxy fatty acids (omega-HFAs), an amination module (Cell-A(m)) to convert terminal alcohol groups of the substrate to amine groups, and a reduction module (Cell-R-m) to convert the carboxyl groups of fatty acids to alcohol groups, were constructed. The product-oriented assembly of these cell modules involving multi-enzymatic cascade reactions generated omega-ADAs (up to 46 mM), alpha,omega-diols (up to 29 mM), omega-amino alcohols (up to 29 mM) and alpha,omega-diamines (up to 21 mM) from 100 mM corresponding FAME substrates with varying carbon chain length (C8, C10, and C12). Finally 12-ADA and 1,12-diol were purified with isolated yields of 66.5% and 52.5%, respectively. The multi-enzymatic cascade reactions reported herein present an elegant 'greener' alternative for the biosynthesis of various biopolymer monomers from renewable saturated fatty acids. | - |
dc.language | 영어 | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | Multi-enzymatic cascade reactions with Escherichia coli-based modules for synthesizing various bioplastic monomers from fatty acid methyl esters | - |
dc.type | Article | - |
dc.identifier.doi | 10.1039/d1gc04532f | - |
dc.citation.journaltitle | Green Chemistry | - |
dc.identifier.wosid | 000758358700001 | - |
dc.citation.endpage | 2231 | - |
dc.citation.number | 5 | - |
dc.citation.startpage | 2222 | - |
dc.citation.volume | 24 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Gee | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
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