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Arecombinant Bifidobacterium bifidum BGN4 strain expressing the streptococcal superoxide dismutase gene ameliorates inflammatory bowel disease
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sini Kang | - |
dc.contributor.author | Zhaoyan Lin | - |
dc.contributor.author | Yang Xu | - |
dc.contributor.author | Minju Park | - |
dc.contributor.author | Geun Eog Ji | - |
dc.contributor.author | Tony V. Johnston | - |
dc.contributor.author | Seockmo Ku | - |
dc.contributor.author | Myeong Soo Park | - |
dc.date.accessioned | 2022-06-13T06:07:06Z | - |
dc.date.available | 2022-06-13T06:07:06Z | - |
dc.date.issued | 2022-06-07 | - |
dc.identifier.citation | Microbial Cell Factories. Vol.21(1):113 | ko_KR |
dc.identifier.issn | 1475-2859 | - |
dc.identifier.uri | https://hdl.handle.net/10371/181284 | - |
dc.description.abstract | Inflammatory bowel disease (IBD) is a gastrointestinal disease characterized by diarrhea, rectal bleeding, abdominal pain, and weight loss. Recombinant probiotics producing specific proteins with IBD therapeutic potential are currently considered novel drug substitutes. In this study, a Bifidobacterium bifidum BGN4-SK strain was designed to produce the antioxidant enzymes streptococcal superoxide dismutase (SOD) and lactobacillus catalase (CAT), and a B. bifidum BGN4-pBESIL10 strain was proposed to generate an anti-inflammatory cytokine, human interleukin (IL)-10. In vitro and in vivo efficacy of these genetically modified Bifidobacterium strains were evaluated for colitis amelioration.
In a lipopolysaccharide (LPS)-stimulated HT-29 cell model, tumor necrosis factor (TNF)-α and IL-8 production was significantly suppressed in the B. bifidum BGN4-SK treatment, followed by B. bifidum BGN4-pBESIL10 treatment, when compared to the LPS-treated control. Synergistic effects on TNF-α suppression were also observed. In a dextran sodium sulphate (DSS)-induced colitis mouse model, B. bifidum BGN4-SK treatment significantly enhanced levels of antioxidant enzymes SOD, glutathione peroxidase (GSH-Px) and CAT, compared to the DSS-only group. B. bifidum BGN4-SK significantly ameliorated the symptoms of DSS-induced colitis, increased the expression of tight junction genes (claudin and ZO-1), and decreased pro-inflammatory cytokines IL-6, IL-1β and TNF-α. These findings suggest that B. bifidum BGN4-SK ameliorated DSS-induced colitis by generating antioxidant enzymes, maintaining the epithelial barrier, and decreasing the production of pro-inflammatory cytokines. Although B. bifidum BGN4-pBESIL10 exerted anti-inflammatory effects in vitro, the enhancement of IL-10 production and alleviation of colitis were very limited. | ko_KR |
dc.language.iso | en | ko_KR |
dc.subject | Inflammatory bowel disease | - |
dc.subject | Bifidobacterium bifidum | - |
dc.subject | Catalase | - |
dc.subject | Superoxide peroxidase | - |
dc.subject | Human interleukin-10 | - |
dc.title | Arecombinant Bifidobacterium bifidum BGN4 strain expressing the streptococcal superoxide dismutase gene ameliorates inflammatory bowel disease | ko_KR |
dc.type | Article | ko_KR |
dc.identifier.doi | https://doi.org/10.1186/s12934-022-01840-2 | ko_KR |
dc.citation.journaltitle | Microbial Cell Factories | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2022-06-12T03:25:24Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 113 | ko_KR |
dc.citation.volume | 21 | ko_KR |
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